Atmospheric Dielectric Barrier Discharge (DBD) plasma development is one of the most interesting subjects to our group. Fridman et al. * has concluded that Floating DBD Direct Plasma are more effective in bactericidal property, it contains higher free charged particles than indirect type. DBD plasma sources can provide low temperature or non-thermal (cold) plasmas, and these plasmas can be created with ambient air or with a noble gas, such as helium or argon, as the carrier medium. It can be used with an electrode as a contact or noncontact method to target a tissue. However, the drawback of direct DBD plasma is that it will work only in short gap distances, on the order of a few millimeters. Our group has investigated -PLASMA Cell Modulation: PhotoBioCare, Thailand). The major area has been focused on its bactericidal disinfection property, application in common dermatologic problems, in skin aesthetic facial rejuvenation and chronic wound management, from 2012 to 2016.
Objective: The objectives of this study were to isolate microorganisms and screen for potential antimicrobial activities from the soil.
Methods:In this study, a total of 425 isolates were isolated from 100 soil samples. The preliminary screening for antimicrobial activities of these isolates was performed by modified cross-streak, agar diffusion, and modified microdilution technique against 16 pathogenic bacteria and fungi.
Results:In the anti-microbial activity, there were three isolates, namely, 277, 303, and 307 exhibited inhibitory activity against methicillin-resistant Staphylococcus aureus and Salmonella typhimurium respectively. This study also examined the various enzymes producing from soil microorganisms including chitinase, chitosanase, amylase, cellulose, caseinase, gelatinase, esterase, and lipase production of different selective media for 24 and 48 hrs using the direct spot method. The results revealed that 28 isolates could produce various enzymes with strong activity. Most of them produced gelatinase (5.65%) and caseinase (5.18%). There were four isolates that produce broad-spectrum enzyme. In addition, the investigation of selected microorganism identification showed that they can be divided into three groups: Burkholderia spp., Pseudomonas spp., and Rhodococcus spp.
Conclusion:This study demonstrated that the microorganisms from soil are capable of producing potential, antibacterial, and bioactive enzymes.
Objective: Our study was to isolate Bacillus spp. from soil all around 6 geographic parts of Thailand and screen for potential antimicrobial.Methods: A total of 43 isolates which isolated from 100 samples of soil were investigated. Preliminary screening was based on antimicrobial activity against 16 strains of pathogenic bacteria and fungi, including 10 strains of Methicillin-resistant Staphylococcus aureus (MRSA), and 1 strain of Methicillin-sensitivity Staphylococcus aureus (MSSA), Extended spectrum beta lactamases (ESBL) Escherichia coli, Samonella Typhymurium, Klebsiella pneumoniae, Candida albicans, Cryptococcus neoformans respectively by cross streak, agar diffusion and modified microdilution technique. Moreover, the selected Bacillus spp. were then screened for bioactive enzyme, including chitinese, chitosanese, amylase, cellulose, caseinase, gelatinase, esterase and lipase production of different selective media for 24 and 48 h by direct spot agar.
Results:There are 2 isolates, namely 23 and 49 showed particularly strong activity inhibitions against MRSA and pathogenic C. albicans. The diameter of hydrolysis zone results from screened for bioactive enzyme revealed that there were 36 isolate showed particularly strong activities with a broad spectrum enzyme. The isolate which produced the widest diameter hydrolysis zone in gelatin, cellulose and starch are 294, 303, and 290 respectively.
Conclusion:Our result indicated Bacillus spp. from soil in Thailand showed potent antibacterial activity and bioactive compounds production.
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