Research of the role of religious belief and/or spirituality has been conducted on a wide range of health-related topics, across many disciplines, and in many countries. The purpose of this study was to determine the relationship between religious beliefs, self-esteem, anxiety, and depression in nursing students in Cyprus. One hundred and twenty-three nursing students were asked to complete a survey consisting of four self-report questionnaires (Beck Depression Inventory, State-Trait Anxiety Inventory, The Royal Free Interview for Religious and Spiritual Beliefs, and Rosenberg Self-esteem Scale). The lowest levels of depression were observed in the third and fourth study year. Normal self-esteem levels were found in the majority of the students (71.3%) and most of them perceived current stress at mild levels. No significant differences on the basis of sex were observed. The vast majority (98.2%) of the students stated a strong religious and/or a spiritual belief that was strongly positively correlated with increased self-esteem and negatively correlated with depression, current stress, and stress as personality trait.