Recent development of non-destructive optical techniques, such as spectroscopy and machine vision technologies, have laid a good foundation for real-time monitoring and precise management of crop N status. However, their advantages and disadvantages have not been systematically summarized and evaluated. Here, we reviewed the state-of-the-art of non-destructive optical methods for monitoring the N status of crops, and summarized their advantages and disadvantages. We mainly focused on the contribution of spectral and machine vision technology to the accurate diagnosis of crop N status from three aspects: system selection, data processing, and estimation methods. Finally, we discussed the opportunities and challenges of the application of these technologies, followed by recommendations for future work to address the challenges.
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