An intramolecular hydrogen bonding directed highly stereospecific, transition metal free synthetic protocol for amide substituted β-aminoenones. High stereoselectivity, excellent yields and high purity were achieved by mere filtration without column chromatographic purification.

In this study, the antimicrobial effect and DNA gyrase inhibitor potential of vanillin-based pyridyl-substituted fluoro-indolines were evaluated. These compounds are synthesized and established through green-chemistry approaches.The inhibition effect on both DNA gyrase A and B was evaluated in silico and in vitro. Agar well diffusion method-based antimicrobial activity against Gram-ve Pseudomonas aeruginosa (MTCC 424) and Escherichia coli (MTCC 443), Gram+ve Streptococcus pyogenes (MTCC 442) and Staphylococcus aureus (MTCC 96), and a clinical isolate of Candida albicans (Fungi) was evaluated.The cytotoxicity of the compounds was assessed over macrophages using the MTT assay. In the results, the target compounds exhibited a broad-spectrum antimicrobial activity against both bacterial types and fungal.