Dans cet article, les 3 organes de C. sieberiana récoltée au Togo ont fait l'objet d'une étude comparative pour la détermination de leur composition phytochimique, teneur en phénols et flavonoïdes totaux, et activités antioxydantes. Le tri phytochimique a été réalisé grâce aux réactions de colorimétrie et précipitation. La teneur en phénols totaux a été déterminée grâce au réactif du Folin-Ciocalteu et celle des flavonoïdes par AlCl 3. L'activité antioxydante a été évaluée par les méthodes de DPPH et FRAP. Les résultats obtenus ont montré que les 3 organes de la plante contiennent des grands groupes chimiques, tels que les flavonoïdes, tanins et saponosides. Les teneurs en phénols totaux des extraits éthanoliques sont (en mg EAG/g) de 276,62 ± 26,120 (feuilles) ; 345,04 ± 3,160 (écorces) ; 327,16 ± 3,990 (racines). Celles des flavonoïdes totaux sont (en mg EQ/g) de 34,134 ± 4,324 (feuilles) ; 36,430 ± 2,022 (écorces) et 37,270 ± 2,216 (racines). Les extraits éthanoliques des 3 organes ont un pouvoir antioxydant appréciable. En conclusion, les résultats obtenus révèlent que les racines et écorces ont des activités antioxydantes semblables, toutefois ne permettent pas à ce stade de la recherche de préciser l'organe approprié pouvant remplacer les racines.
Well established as source of bioactive compounds, medicinal plants have long been used in treatment of human ailments. The validation of traditional use of medicinal plants can be achieved by screening a collection of extracts for bioactivity using in vitro, in cellulo and even in vivo assays. The results of this work are consistent with the traditional use of the various parts of Ficus sur to cure hepatic pain, cardiovascular and neurodegenerative diseases, cancer, and diabetes. Although the bark extract is more active than the extracts of the other organs, it would be better to use the leaves to preserve the plant, as these contain all the metabolites present in the bark and much more. Please cite this paper as:Saloufou KI, Boyode P, Simalou O, Eloh K, Idoh K, Melila M, et al. Chemical composition and antioxidant activities of different parts of Ficus sur.Introduction: Ficus sur is a plant widely used in traditional pharmacopoeia in Togo. So, this study aimed the assessment of antioxidant properties and identification of some compounds from the ethanolic extracts of different parts of the plant (leaves, fruits, roots, and barks). Methods: The phenolic and flavonoid contents of the ethanolic extracts of different organs of Ficus sur were assessed using conventional known methods. The DPPH radical scavenging and the ferric-reducing antioxidant power (FRAP) assays were used to highlight the antioxidant activities. The different extract samples were also analysed by liquid chromatography coupled to a quadrupole-time of flight mass detector (ESI-QTOF). Results: Total phenolic contents (TPCs) for 1 mg of dry extract ranged from 489.40 ± 7.48 μg GAE (gallic acid equivalents) for the bark to 62.34 ± 2.66 μg GAE for unripe fruits. The bark exhibited the highest flavonoid content, which was closed to 90.20 ± 3.72 μg QE (quercetin equivalents)/mg of dry extract. The radical scavenging activities of the bark and unripe fruits were 56.50 ± 0.29 and 7.3 ± 0.30 μg QE/mg of dry extract, respectively. In the same order, the FRAP values of the two organs were 104.57 ± 4.75 and 19.61 ± 0.22 μmol FeSO 4 Eq/mg of dry extract. Many compounds including notoginsenoside R10; 4' ,5,7-trihydroxyflavan-3-ol; catechin; and boviquinone 4 were identified. Conclusion: The various organs of Ficus sur are a source of bioactive compounds especially phenolic compounds and flavonoids with antioxidant activity.
Holarrhena floribunda trunk bark exhibited antihypertensive and antioxidant properties, supporting its use for the treatment of hypertension in traditional medicine. So, more investigations are needed for identification of the active compounds in Holarrhena floribunda ethanolic extract involved in antioxidant and antihypertensive activities.
In Togo, the abusive use of the root of Cassia sieberiana D.C. in traditional medicine, contributes gradually to the rarefaction of the species. The general objective of this study is to promote the use of vital organs of Cassia sieberiana in traditional medicine in Togo. The identification of secondary metabolites of the extracts (cyclohexane, dichloromethane and methanol) was carried out by GC-MS and by CL-MS / MS. The antibiotic susceptibility test was performed according to the well diffusion method and the MICs and MBCs according to the tube dilution method. Compounds such as sitosterol α-acetate, β-sitosterol, emodin, chaetochromine, luteolin, (±) -catechin, naringenin 5-O-rhamnoside, guibourtinidol- (4 alpha-> 6) -catechin and (-) - epiafzelechin are found in the root and in the stem bark. The identified molecules give the different methanolic extracts, an antibacterial effect on all the germs tested. At the end of this study, it appears that the chemical composition of the stem bark is almost similar to that of the root bark. The leaves would be better placed for the treatment of bacteria tested.
Tel. : (+228) 91 58 81 61 REMERCIEMENTS Nos sincères remerciements vont à l'Agence Universitaire de la Francophonie (AUF) pour le financement de ce travail. RESUMEFicus sur est une plante alimentaire couramment utilisée en médecine traditionnelle au Togo. Elle a cependant fait l'objet de très peu d'études phytochimiques. Le présent travail a pour but de valoriser cette espèce à travers l'identification continue de ses métabolites. Ainsi, l'extraction cyclohexanique au Soxhlet a été réalisée sur les feuilles de Ficus sur. Cet extrait cyclohexanique obtenu a été ensuite fractionné puis purifié par chromatographie sur colonne de gel de silice. L'identification des composés a été réalisée par GC-MS, précédée quelquefois d'une étape de dérivatisation. Treize fractions (A1 à A13) ont été obtenues du fractionnement de cet extrait. Après deux purifications successives de la fraction A5, deux sous-fractions A5. Identification of two bioactive phytosterols of the cyclohexane extract of Ficus sur (moraceae) leaves ABSTRACTFicus sur is a food plant commonly used in traditional medicine in Togo. However, few phytochemical studies of this species are available. This work aims at contributing to the enhancement of this approach through an identification of its metabolites. The leaves of Ficus sur were then extracted with cyclohexane using soxhlet. The obtained cyclohexane extract was fractionated and purified by chromatography on silica gel column. Identification of the compounds was carried out by GC-MS, sometimes preceded by a derivatization step. Thirteen fractions (A1 to A13) were obtained from the fractionation of this extract. After two successive purifications of the fraction A5, two subfractions A5.1 and A5.2 were obtained. The mass spectrum of a compound of A2 has a molecular peak at m/z 426 and peaks [M-69]
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