Light induces phosphorylation of photosystem II (PSII) proteins in chloroplasts by activating the protein kinase(s) via reduction of plastoquinone and the cytochrome b6f complex. The recent finding of high-light-induced inactivation of the phosphorylation of chlorophyll a͞b-binding proteins (LHCII) of the PSII antenna in floated leaf discs, but not in vitro, disclosed a second regulatory mechanism for LHCII phosphorylation. Here we show that this regulation of LHCII phosphorylation is likely to be mediated by the chloroplast ferredoxin-thioredoxin system. We present a cooperative model for the function of the two regulation mechanisms that determine the phosphorylation level of the LHCII proteins in vivo, based on the following results: (i) Chloroplast thioredoxins f and m efficiently inhibit LHCII phosphorylation. (ii) A disulfide bond in the LHCII kinase, rather than in its substrate, may be a target component regulated by thioredoxin. (iii) The target disulfide bond in inactive LHCII kinase from dark-adapted leaves is exposed and easily reduced by external thiol mediators, whereas in the activated LHCII kinase the regulatory disulfide bond is hidden. This finding suggests that the activation of the kinase induces a conformational change in the enzyme. The active state of LHCII kinase prevails in chloroplasts under low-light conditions, inducing maximal phosphorylation of LHCII proteins in vivo. (iv) Upon high-light illumination of leaves, the target disulfide bond becomes exposed and thus is made available for reduction by thioredoxin, resulting in a stable inactivation of LHCII kinase. R eversible protein phosphorylation is a unique regulatory mechanism for modification of the structure and function of proteins in both prokaryotic and eukaryotic organisms. It plays a fundamental role in signal transduction pathways that relay information from outside of the cell to the gene level. Bennett (1, 2) discovered the reversible, light-dependent phosphorylation of proteins in thylakoid membranes of plant chloroplasts. All thylakoid phosphoproteins identified so far are closely associated with photosystem II (PSII), a light-driven waterplastoquinone-oxidoreductase enzyme. Four core proteins of PSII, including the D1 and D2 reaction center proteins, the 43-kDa chlorophyll a-binding protein (CP43 protein), and the psbH gene product (PsbH protein) are prone to redox-regulated reversible phosphorylation. Three of six chlorophyll a͞b-binding proteins of the PSII antenna, Lhcb1 and Lhcb2 (designated LHCII) as well as Lhcb4 proteins, also undergo light-dependent phosphorylation. The protein kinase(s) involved in phosphorylation of the PSII proteins is associated with thylakoid membranes (2) and is activated by light via reduction of electron transfer components, plastoquinone and cytochrome b 6 ͞f complex (2-6).A large majority of the experimental data support the existence of two different kinases for PSII phosphoproteins, one for LHCII and another for PSII core proteins with distinct redox regulation systems (3, 6-10). Th...

Light-induced phosphorylation of light-harvesting chlorophyll a/b complex II (LHCII) proteins in plant thylakoid membranes requires an activation of the LHCII kinase via binding of plastoquinol to cytochrome b 6 f complex. However, a gradual down-regulation of LHCII protein phosphorylation occurs in higher plant leaves in vivo with increasing light intensity. This inhibition is likely to be mediated by increasing concentration of thiol reductants in the chloroplast. Here, we have determined the components involved in thiol redox regulation of the LHCII kinase by studying the restoration of LHCII protein phosphorylation in thylakoid membranes isolated from high-light-illuminated leaves of pumpkin (Cucurbita pepo), spinach (Spinacia oleracea), and Arabidopsis. We demonstrate an experimental separation of two dynamic activities associated with isolated thylakoid membranes and involved in thiol regulation of the LHCII kinase. First, a thioredoxin-like compound, responsible for inhibition of the LHCII kinase, became tightly associated and/or activated within thylakoid membranes upon illumination of leaves at high light intensities. This reducing activity was completely missing from membranes isolated from leaves with active LHCII protein phosphorylation, such as dark-treated and low-light-illuminated leaves. Second, hydrogen peroxide was shown to serve as an oxidant that restored the catalytic activity of the LHCII kinase in thylakoids isolated from leaves with inhibited LHCII kinase. We propose a dynamic mechanism by which counteracting oxidizing and reducing activities exert a stimulatory and inhibitory effect, respectively, on the phosphorylation of LHCII proteins in vivo via a novel membrane-bound thiol component, which itself is controlled by the thiol redox potential in chloroplast stroma.Light induces the phosphorylation of a number of photosystem II (PSII)-related proteins in the thylakoid membranes of plant chloroplasts (Bennett, 1977(Bennett, , 1991, including two light-harvesting chlorophyll a/b complex II (LHCII) proteins, Lhcb1 and Lhcb2, of the PSII outer antenna (Larsson et al., 1987). Phosphorylation of LHCII proteins has been proposed to balance the excitation energy between PSII and photosystem I (PSI) in plant thylakoid membranes (Bennett, 1991;Allen, 1992). The phosphorylation of LHCII proteins is regulated in response to light via activation of the LHCII kinase with reduced plastoquinone pool and cytochrome b 6 f complex (Cyt b 6 f; Vener et al., 1995Vener et al., , 1997Gal et al., 1997;Zito et al., 1999). Moreover, LHCII protein phosphorylation is down-regulated under high light conditions in vivo, revealing the existence of an inhibitory control mechanism in chloroplasts (Rintamäki et al., 1997). We have recently shown that the chloroplast thioredoxins f and m are effective inhibitors of LHCII protein phosphorylation in vitro (Rintamäki et al., 2000). It has become apparent that the correct thiol redox state is critical for in vitro thylakoid protein phosphorylation (Carlberg et al., 1999;Rinta...

Differential redox regulation of thylakoid phosphoproteins was studied in winter rye plants in vivo . The redox state of chloroplasts was modulated by growing plants under different light/temperature conditions and by transient shifts to different light/temperature regimes. Phosphorylation of PSII reaction centre proteins D1 and D2, the chlorophyll a binding protein CP43, the major chlorophyll a / b binding proteins Lhcb1 and Lhcb2 (LHCII) and the minor lightharvesting antenna protein CP29 seem to belong to four distinct regulatory groups. Phosphorylation of D1 and D2 was directly dependent on the reduction state of the plastoquinone pool. CP43 protein phosphorylation generally followed the same pattern, but often remained phosphorylated even in darkness. Phosphorylation of CP29 occurred upon strong reduction of the plastoquinone pool, and was further enhanced by low temperatures. In vitro studies further demonstrated that CP29 phosphorylation is independent of the redox state of both the cytochrome b 6 /f complex and the thiol compounds. Complete phosphorylation of Lhcb1 and 2 proteins, on the contrary, required only modest reduction of the plastoquinone pool, and was subject to inhibition upon increase in the thiol redox state of the stroma. Furthermore, the reversible phosphorylation of Lhcb1 and 2 proteins appeared to be an extremely dynamic process, being rapidly modulated by short-term fluctuations in chloroplast redox conditions.