Many freshwater environments experience dramatic seasonal changes with some systems remaining ice-covered for most of the winter. Freshwater systems are also highly sensitive to environmental change. However, little is known about changes in microbial abundance and community composition during lake ice formation and times of persistent ice cover. The goal of this study is to characterize temporal dynamics of microbial communities during ice formation and persistent ice cover. Samples were collected in triplicate, five days per week from surface water in the Keweenaw Waterway between November and April. Environmental conditions along with microbial abundance and microbial community composition was determined. Distinct community composition was found between ice-free and ice-covered time periods with significantly different community composition between months. The microbial community underwent dramatic shifts in microbial abundance and diversity during the transitions into and out of ice cover. The richness of the microbial community increased during times of ice cover. Relatives of microbes involved in nitrogen cycling bloomed during times of ice cover as sequences related to known nitrifying taxa were significantly enriched during ice cover. These results help to elucidate how microbial abundance and diversity change over drastic seasonal transitions and how ice cover may affect microbial abundance and diversity.
Many aquatic environments are at risk for oil contamination and alkanes are one of the primary constituents of oil. The alkane hydroxylase (AlkB) is a common enzyme used by microorganisms to initiate the process of alkane-degradation. While many aspects of alkane bioremediation have been studied, the diversity and evolution of genes involved in hydrocarbon degradation from environmental settings is relatively understudied. The majority of work done to-date has focused on the marine environment. Here we sought to better understand the phylogenetic diversity of alkB genes across marine and freshwater settings using culture-independent methods. We hypothesized that there would be distinct phylogenetic diversity of alkB genes in freshwater relative to the marine environment. Our results confirm that alkB has distinct variants based on environment while our diversity analyses demonstrate that freshwater and marine alkB communities have unique responses to oil amendments. Our results also demonstrate that in the marine environment, depth is a key factor impacting diversity of alkB genes.
Aims: This study explores the microbial diversity of sources which may influence boat microbial communities. We investigated the impact of dock, air and water microbial communities on the hull, transom and bilge microbial communities over the span of 11 days. Methods and Results: Using source tracking software, we investigated the extent to which each of our potential sources (air, water and dock) influenced the overall microbial community. This study concluded that the dock impacted 14-64% of the hull and transom microbial community. Micro-organisms from the water were shown to impact 5Á6% the bilge microbial community but had minimal impact on hull and transom microbial communities. Micro-organisms from the air had minimal impact in all areas of the boat. Conclusions: Our results demonstrate that micro-organisms from sources other than water can influence the microbial community of a boat, suggesting that terrestrial micro-organisms can impact the boat microbial community. Significance and Impact of the Study: Outside of ballast tanks, microbial diversity on boats is largely unexplored. While ballast water is widely recognized as a route for dispersal of allochthonous micro-organisms, comparatively little is known about the microbial diversity on other areas of the boat. If the organisms on a boat originate from sources other than water, there is potential that terrestrial micro-organisms could be dispersed by shipping activity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.