Table S1. List of the mollusc species collected in the survey between Cabopino and Calaburras, Málaga province, Spain. T1: transect off Torre de Calahonda; T2: transect off Torre Pesetas, T3: transect west of the river "Cala del Moral"; T4: transect west of "Cabezo del Fraile". The numbers given for each sampling point are the total live collected specimens for all replicates and all seasonal sampling campaigns.
This unit describes the use of PCR to construct hybrid DNA molecules. The unit provides an overview of how PCR can be exploited to accomplish numerous cloning strategies. The Basic Protocol outlines the PCR amplification and cloning strategies. The Commentary includes a troubleshooting guide for problems most frequently encountered in PCR cloning, plus four specific examples of the application of this technique to create in-frame fusion proteins, to create recombinant DNA products, to generate deletions and inversions by inverse PCR, and to introduce mutagenized PCR products or specific mutations or fusions by gap repair in yeast.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.