Experiments were performed to extract low molecular weight peptides from the eggs of the sea urchin Strongylocentrotus pallidus using sterilizing microfiltration and solid-phase extraction, along with analytical gel chromatography and spectroscopy. The peptides extracted had molecular weights of 360 -1200 Dal and were divided into two fractions: acidic peptides contained a predominance of glutamic and aspartic acid residues, along with aromatic amino acid residues; alkaline peptides contained mainly lysine and arginine residues and lacked aromatic amino acids. Assessment of the biological activity of these extracts demonstrated high specificity in stimulating the development of explants in organotypic cultures of spleen, testicle, myocardium, and cerebral cortex tissues.
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