In recent times, nanoparticles are attributed to green nanotechnology methods to know the synergistic biological activities. To accomplish this phenomenon, present study was aimed to synthesize copper oxide nanoparticles (CuO NPs) by using Syzygium alternifolium stem bark, characterized those NPs using expository tools and to elucidate high prioritized antimicrobial and anticancer activities. Synthesized particles exhibited a color change pattern upon synthesis and affirmed its respective broad peak at 285 nm which was analyzed through UV-vis spectroscopy. FT-IR study confirmed that phenols and primary amines were mainly involved in capping and stabilization of nanoparticles. DLS and Zeta potential studies revealed narrow size of particles with greater stability. XRD studies revealed the crystallographic nature of particles with 17.2 nm average size. Microscopic analysis by using TEM revealed that particle size range from 5-13 nm and most of them were spherical in shape, non-agglomerated and poly-dispersed in condition. Antimicrobial studies of particles showed highest inhibitory activity against E. coli and T. harzianum among bacterial and fungal strains, respectively. The scope of this study is extended by examining anticancer activity of CuO NPs. This study exhibited potential anticancer activity towards MDA-MB-231 human breast cancer lines. Overall, these examinations relate that the S. alternifolium is described as efficient wellbeing plant and probabilistically for the design and synthesis of nanoparticles for human health. This study paves a way to better understand antimicrobial and anticancer therapeutic drug potentials of nanoparticles to design and analysis of pharmaceuticals by in vivo and in vitro approaches.
The efficiency of two methods of pretreatment (NaOH and H2O2) on lignocelluloses—saw dust, wheat straw, sugarcane bagasse and rice bran—was compared in the present study. Alkali treatment of lignocelluloses relatively removed more hemicelluloses and lignin leaving behind cellulose content in the residues than peroxide treatment. Crude cellulase of Aspergillus niger, produced on the pretreated sawdust with highest cellulose content, was further tested for the release of soluble and reducing sugars during the saccharification process of same pretreated saw dust. The saccharification process of the pretreated sawdust with enzyme was optimized for pH, temperature, and substrate concentration and proceeded optimally at pH of 5.0, 50 °C and 0.5 % pretreated sawdust. The rate of saccharification with crude enzyme of A. niger on alkali-treated sawdust was found to be maximum (23 %) as against 5.4 % on native sawdust under optimal conditions after 48 h. The present study indicates NaOH-treated sawdust as a potential raw material for both production of cellulase and saccharification in a large scale.
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