Diamlne oxidase or its substrates can be measured using a coupled reaetion with peroxidase and the ehromogen 3-methyl-2-benzothiazolone hydrazone (MBTH) with an appropriate aeeeptor such as 3-(dimethylamino)henzoic acid (DMAB). This method provides a more rapid and sensitive method of measuring histamine than current colorimetrle assays; using this system, histamine was quantltated at eoneentrations of 10 to 400/~mol]l. The Km of DAO was determined to be 2.9 x 10 -5 M, a lower value than previously reported.
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