Background: Chickpea is a nutritional rich premier pulse crop but its production encounters setbacks due to various stresses and understanding of molecular mechanisms can be ascribed foremost importance. Objective: The investigation was carried out to identify the differentially expressed WRKY TFs in chickpea in response to herbicide stress and decipher their interacting partners. Methods: For this purpose, transcriptome wide identification of WRKY TFs in chickpea was done. Behavior of the differentially expressed TFs was compared between other stress conditions. Orthology based cofunctional gene networks were derived from Arabidopsis. Gene ontology and functional enrichment analysis was performed using Blast2GO and STRING software. Gene Coexpression Network (GCN) was constructed in chickpea using publicly available transcriptome data. Expression pattern of the identified gene network was studied in chickpea-Fusarium interactions. Results: A unique WRKY TF (Ca_08086) was found to be significantly (q value = 0.02) upregulated not only under herbicide stress but also in other stresses. Co-functional network of 14 genes, namely Ca_08086, Ca_19657, Ca_01317, Ca_20172, Ca_12226, Ca_15326, Ca_04218, Ca_07256, Ca_14620, Ca_12474, Ca_11595, Ca_15291, Ca_11762 and Ca_03543 were identified. GCN revealed 95 hub genes based on the significant probability scores. Functional annotation indicated role in callose deposition and response to chitin. Interestingly, contrasting expression pattern of the 14 network genes was observed in wilt resistant and susceptible chickpea genotypes, infected with Fusarium. Conclusion: This is the first report of identification of a multi-stress responsive WRKY TF and its associated GCN in chickpea.
Chickpea is one of the major pulse crops cultivated worldwide. It is affected by many fungal diseases including rust caused by the fungus Uromyces ciceris arietini. Its severity is mainly observed during the owering and pod development stage and this causes considerable yield losses. The present investigation was carried out at ICAR-NIASM, Baramati during 2021-2022 to assess genotypic variation in stem reserve mobilization (SRM) and its in uence on loss of grain yield due to rust infection on the basis of severity of rust, seed yield and test weight. The SRM was strongly correlated (R 2 0.96) with yield irrespective of the severity of rust infection. Phule Vikram, a local chickpea genotype, recorded the highest grain yield (1506.6 kg/ha) and SRM (23.65 mg/g stem dry weight) while the lowest yield was recorded in Pusa Green 112 (476.7 kg/ha), a susceptible, genotype that had less SRM (3.44 mg/g stem dry weight) and partially lled grain. The results indicated that SRM has a major role in ensuring pod lling even under rust infection. Hence, it is suggested that the emphasis should be on SRM as a trait for selection of early generation breeding lines in the chickpea improvement program with precaution to reduce a penalty if any in terms of attraction of pests by increased access to soluble sugars in high SRM genotypes.
Chickpea is one of the major pulse crops cultivated worldwide. It is affected by many fungal diseases including rust caused by the fungus Uromyces ciceris arietini. Its severity is mainly observed during the flowering and pod development stage and this causes considerable yield losses. The present investigation was carried out at ICAR-NIASM, Baramati during 2021–2022 to assess genotypic variation in stem reserve mobilization (SRM) and its influence on loss of grain yield due to rust infection on the basis of severity of rust, seed yield and test weight. The SRM was strongly correlated (R2꞊ 0.96) with yield irrespective of the severity of rust infection. Phule Vikram, a local chickpea genotype, recorded the highest grain yield (1506.6 kg/ha) and SRM (23.65 mg/g stem dry weight) while the lowest yield was recorded in Pusa Green 112 (476.7 kg/ha), a susceptible, genotype that had less SRM (3.44 mg/g stem dry weight) and partially filled grain. The results indicated that SRM has a major role in ensuring pod filling even under rust infection. Hence, it is suggested that the emphasis should be on SRM as a trait for selection of early generation breeding lines in the chickpea improvement program with precaution to reduce a penalty if any in terms of attraction of pests by increased access to soluble sugars in high SRM genotypes.
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