No abstract

Investigation of putative allergens from tomato berries is challenging as differences between human serum IgE specifi city and reactivity as well as the non-specifi c binding of the primary and secondary antibodies often cause diffi culties. In this study fi ve tomato cultivars were investigated to evaluate their potential allergenicity in Hungarian tomato sensitive patients. The major allergens proved to be low molecular weight proteins, but several previously described allergens could be identifi ed as well using IgE-Western blotting. IgE binding to cross-reactive carbohydrate determinants (CCDs) was ruled out through the use of CCD inhibitor, but non-specifi c binding of the secondary antibody remained an issue. IgE binding activity of a purifi ed, immunoblot positive protein (band at 40 kDa), and non-specifi c binding of the secondary antibody to the same protein, was demonstrated with an Optical Waveguide Lightmode Spectroscopy (OWLS) based immunosensor. LC-ESI-MS/MS analysis showed this protein is an as-yet undescribed vicilin-type putative allergen.

No abstract

The aim of the present work was to evaluate the influence of genetic impact on the content of some characteristic secondary metabolites in tomato fruits. The study was conducted to screen 14 different tomato genotypes for antioxidant capacity and quality attributes (polyphenols, tocopherols, carotenoids, vitamin C) to select the genetic lines with high biological value, multiple disease resistance, and high yield capacity for further usage in crop improvement programs. Significant differences were found among the different hybrids and cultivation seasons for the phytochemical content of the fruits. The varieties developed by breeding to increase their resistance were found to differ significantly. During a 3-year cultivation experiment, the level of lycopene in freshly harvested fruits ranged between 10.38 and 63.23 mg.kg−1 fwt for outdoor-cultivated Naik114 and Naik352, respectively. As for β-carotene, its content varied between 0.49 mg.kg−1 for Unorosso and 11.66 mg.kg−1 for Cherrola F1. The highest level of vitamin C (702.59 mg.kg−1) was recorded for Cherrola F1, while the lowest level (1.36.86 mg.kg−1) was determined in fruits of Unorosso. Neither polyphenol nor vitamin C showed positive correlation with antioxidant activity. In the three cultivation seasons, the highest concentration of polyphenols (579.19–804.12 mg.kg−1 fwt) was determined in fruits of outdoor-cultivated Cherolla F1 variety. The α- and γ-tocopherol content varied between 2.56 and 18.99 mg.kg−1, and 0.28 and 9.35 mg.kg−1, respectively, in fresh tomato fruit. Among the newly bred resistant varieties, the ZKI114 variety was proved to have outstanding features concerning the amounts of the bioactive components.

No abstract

A three-year (2016–2018) open field experiment was conducted to study the effect of irrigation, fertilisation, and seasonal variation on the main bioactive components, such as carotenoids (lycopene and β-carotene), total polyphenols, antioxidant capacity, and tocopherols of processed Uno Rosso F1 tomato. The statistical evaluation of measurements proved that the multi-year data set cannot be evaluated as combined data set; the values obtained in different years must be evaluated separately. The impact of irrigation on the content of bioactive components varied from year to year. The correlation was negative between irrigation and α-tocopherol content in 2016 and 2018 (r = –0.567 and –0.605, respectively), polyphenol content in 2016 (r = –0.668), γ-tocopherol content in 2017 (r = –0.662), while positive correlation was observed between concentration of vitamin C (r = 0.533) in 2017, lycopene content (r = 0.473) in 2018 and irrigation intensity. A weak correlation was proved between K levels and concentrations of lycopene and polyphenols in 2016 (r = 0.301 and r = 0.392, respectively).