We describe how the James Webb Space Telescope (JWST) Near-Infrared Spectrograph's (NIRSpec's) detectors will be read out, and present a model of how noise scales with the number of multiple non-destructive reads samplingup-the-ramp. We believe that this noise model, which is validated using real and simulated test data, is applicable to most astronomical near-infrared instruments. We describe some non-ideal behaviors that have been observed in engineering grade NIRSpec detectors, and demonstrate that they are unlikely to affect NIRSpec sensitivity, operations, or calibration. These include a HAWAII-2RG reset anomaly and random telegraph noise (RTN). Using real test data, we show that the reset anomaly is: (1) very nearly noiseless and (2) can be easily calibrated out. Likewise, we show that large-amplitude RTN affects only a small and fixed population of pixels. It can therefore be tracked using standard pixel operability maps.
The importance of groundwater in the dissolved organic matter (DOM) budget of small upland streams is not well understood. This paper is concerned with the amount of streamflow which can be attributed to groundwater, the organic chemistry of rainwater, streamwater, and groundwater, and the rate of transfer of DOM in groundwater to the streams of a small mountain catchment basin in Alberta. Using naturally occurring isotopes (80 and tritium) groundwater is concluded to be the largest contributor to stream discharge throughout the year. This means that most of the water which reaches the stream must pass through the soil column and be exposed to microbial attack. Groundwater in the Marmot Basin spends an average of about ten years in the ground before being discharged into streams. In this area it appears that the majority of DOM from forest productivity is consumed in the soil and only small amounts of refractory by-products reach the stream. This is in keeping with the finding of Fisher & Likens (1973) that 99% of forest productivity is consumed terrestrially. It is probable that bacteria in stream sediments are capable of taking up refractory compounds which deep soil bacteria can not. Increases in DOM concentration in streams are not usually observed during storm runoff because of the ability of bacteria to take up groundwater DOM and because most of stream discharge is groundwater low in DOM being flushed into the channel even during snowmelt and rainfall events.
This study was conducted to assess the prevalence and zoonotic potential of giardiasis in domestic ruminants. Prevalence of infection was 17.7% in sheep and 10.4% in cattle and was significantly higher in lambs and calves (35.6% and 27.7%, respectively). Naturally infected lambs released cysts intermittently for months. Giardia trophozoites from sheep had typical claw hammer-shaped median bodies and were successfully cultured in TYI-S-33 medium, and cytosolic, cytoskeletal, and membrane fractions exhibited protein profiles similar to human isolates (WB). Immunoblotting showed that sera from infected sheep recognized human Giardia, sera from patients with giardiasis recognized Giardia from sheep, and in both cases recognition involved antigenic proteins of similar molecular weight. Cyst output and clinical signs in ovine infection resemble human disease and the organisms infecting humans and ruminants are morphologically and antigenically similar. It is postulated that domestic ruminants may be a reservoir for human infection and vice versa, thus classifying giardiasis as a zooanthroponotic disease.
The emerging concept of host specificity of Cryptosporidium spp. was exploited to characterize sources of fecal contamination in a watershed. A method of molecular forensic profiling of Cryptosporidium oocysts on microscope slides prepared from raw water samples processed by U.S. Environmental Protection Agency Method 1623 was developed. The method was based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple species/genotypes of Cryptosporidium in a single water sample.
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