It was shown recently that microorganisms can grow in refrigerated braunschweiger liver sausage stuffed in Visking and covered with Pliofilm (2). The organisms grew most abundantly in the outer area of the sausage and it was concluded that they were stimulated by oxygen absorbed through the double casing. This work has now been extended to include other plastic films.
METHODSSmall liver sausages approximately 2.5 inches in diameter and 6 inches long were stuffed in different artificial casings. Methods of sampling and culturing were essentially the same as those already described ( 2 ) . Samples were taken from 3 areas or zones of each sausage, zone A being in the center, zone B midway between the center and the outside, and zone C next to the casing. Appropriate dilutions of all samples were cultured in: Difco tryptone glucose extract agar plates and Difco potato dextrose acid agar plates incubated aerobically a t 30°C.(86"F.) for 5 days; BBL thioglycollate agar plates incubated anaerobically a t 3OoC.(86"F.) for 5 days; and Difco yeast dextrose agar shake tubes incubated anaerobically a t 5"C.(4loF.) for 30 days.Hydrogen ion concentration was determined by the method described by Watson (3) for Swiss cheese. Nitrogen was measured by the micro-Kjeldahl procedure of Johnson ( 1 ) .
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