In Saccharomyces cerevisiae, lack of nutrients triggers a pleiotropic response characterized by accumulation of storage carbohydrates, early Gl arrest, and sporulation of a/at diploids. This response is thought to be mediated by RAS proteins, adenylate cyclase, and cyclic AMP (cAMP)-dependent protein kinases. This study shows that expression of the S. cerevisiae gene coding for a cytoplasmic catalase T (CTTI) is controlled by this pathway: it is regulated by the availability of nutrients. For growth on STMD2%, cells grown on YPD2% plates or in liquid YPD2% were transferred to STMD2% (optical density at 600 nm, 1.0) and were incubated at 30°C for 36 h (after this period, all cells were arrested unbudded in early Gl). Synthetic a-factor (kindly provided by K; Nasmyth) was added in some experiments to a final concentration of 0. 1 p.g per ml of culture. Cells were grown and incubated on SM in the same way as on STMD2%. Strains bearing cdc mutations were pregrown at 23°C on YPD2% to an optical density at 600 nm of 2.5, and the cultures were divided, transferred to STMD2% in some experiments, and incubated for 5 h at 23 and 35°C on YPD2% or STMD2%, respectively, before being harvested.Enzyme activities. The specific activity of ,-galactosidase of crude extracts from yeast strains bearing a single-copy CTTI-lacZ fusion gene (36) integrated into the chromosomal URA3 locus (see Fig. 1) was assayed as described by Miller (26).
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