A study was carried out in four vifiages of Makenene Sub-Division some 85 km north-west of Yaounde, between November 1993 and March 1994 to assess the prevalence and intensity of schistosomes and other helminth infections among senior primary school pupils aged 9–16. An extensive questionnaire was used to collect sociodemographic information and information on water-related activities.The overall prevalence of S. mansoni infection was 228 (23.6%) while S. haematobium infection was practically non-existent 03 (<1%). Infection rates with intestinal nematodes were very high, up to 98% in some villages. Prevalence rates of schistosomiasis varied significantly (P<0.05) between vifiages with the highest (36.2%) recorded in Makenene. Infections with other parasite species encountered were significantly different (P<0.01) while a marked association was observed between water contact activities and the distribution of S. mansoni infections. However, S. mansoni infection intensities were not significantly different (P>0.05) between those with a history of previous infection and treatment and those without.

Consistent performance of anti-drug antibody (ADA) assays through all stages of clinical development is critical for the assessment of immunogenicity and interpretation of PK, PD, safety, and efficacy. The electrochemiluminescent assays commonly employed for ADA measurement use drug conjugated with ruthenium and biotin to bind ADA in samples. Here we report an association between high nonspecific ADA responses in certain drug-naïve individuals and the storage buffer of the conjugated reagents used in a monoclonal antibody ADA assay. Ruthenylated reagents stored in phosphate-buffered saline (PBS) buffer had increased levels of aggregate and produced variable and high baseline responses in some subjects. Reagents stored in a histidine-sucrose buffer (HSB) had lower aggregate levels and produced low sample responses. In contrast to PBS, conjugated reagents formulated in HSB remained low in aggregate content and in sample response variability after 5 freeze/thaw cycles. A reagent monitoring control (RMC) serum was prepared for the real-time evaluation of conjugated reagent quality. Using appropriate buffers for storage of conjugated reagents together with RMCs capable of monitoring of reagent aggregation status can help ensure consistent, long-term performance of ADA methods.

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Summary :Two regions of the Jos Metropolis in Plateau State, Nigeria, with contrasting topographic features and harbouring many snails infes

A preliminary parasitological and malacological survey was effected in rural communities of some Local Government Areas (LGA) in Plateau State, Nigeria, to estimate the prevalence of urinary schistosomiasis and identify active transmission foci. Out of 2888 persons examined in six LGAs, 1381 (47.82%) were excreting eggs of Schistosoma haematobium in their urine. Prevalence rates did not vary significantly (P > 0.05) between the LGAs: Pankshin (62.4%), Shendam (40.2%), Qua'an-Pan (22.9%), Langtang South (45.4%), Langtang North (58.8%) and Wase (50.0%). Infection rates were significantly different (P < 0.001) between the sexes. Many water bodies in the study communities were colonized by infected Bulinus snails. Snail infection rates varied significantly (P < 0.001) between the dry and wet seasons. A positive correlation was observed between snail infection rates and the prevalence of S. haematobium.