SUMMARY A patient is reported with chronic abdominal pain, diarrhoea, and associated radiological and endoscopic abnormalities of the sigmoid colon. Light and electron microscopic study of colorectal mucosa showed abnormal collagenous thickening of the subepithelial basement membrane. The authors felt that the clinical and morphological features justified a diagnosis of collagenous colitis. Review of the literature suggested that collagenous colitis was still an unrecognised entity. Lindstrom (1976) recorded the first case of an hitherto undescribed entity for which he suggested the term 'collagenous colitis'. Lindstrom's report concerned a 48 year old woman with chronic watery diarrhoea; a rectal biopsy showed a heavy collagenous deposit in the mucosal basement membrane. To our knowledge, no further description of this singular entity has appeared in the literature.We
Introduction. Listeriolysin O (LLO), a pore-forming citolysin is a key virulence factor of Listeria monocytogenes (LM). LLO induces a wide range of biological effects during the infection by LM or by itself as recombinant antigen. Here we studied the effects of a non hemolytic form of LLO on the production of two key cytokines for coordinating innate and adaptive immune responses against intracellular pathogens such as IL-12 and IL-23. Objective. We aimed to test the innate immune response of Human Monocyte-derived Dendritic cells (hDCs) to a non-hemolytic form of LLO named as dtLLO (mutated at C484A, W491A, W492A positions). Materials and methods. hDCs were stimulated with dtLLO ( 1μg/mL), in the presence or absence of 50 μg/mL of polymyxin B; 24 hours after stimulation, sups were tested to measure the concentration of TNF-α, IL-6, IL-10, IL-12 and IL-23 by ELISA technique. Results: dtLLO induced the production of IL-6 (1,660.86 pg/mL), TNF α (2,732.36 pg/mL), IL-12 (2,242.92 pg/mL) IL-10 (930.66 pg/mL) and IL-23 (349.47 pg/mL). Cytotoxic effects were undetected. Conclusion: We have identified a potent innate immune response when dtLLO is used as an exogenous antigen on hDCs. The production of IL-12 and IL-23 by human DCs after the recognition of LLO could be very important for the immune response during the human infection by Listeria. As a potential adjuvant for human vaccination purpose, dtLLO could be use to polarized the immune responses toward a Th1 or Th17 responses.
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