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The aim of this study was to characterise flavour differences between short-ripened Gouda-type cheeses produced from raw and pasteurised milk and to follow, in the case of pasteurised milk cheeses, flavour evolution during ripening. The volatile composition of the cheeses was studied using a combination of simultaneous steam distillation-extraction and gas chromatography-mass spectrometry. Visualisation of the analytical results was performed by principal component analysis. A clear differentiation between raw milk cheese and pasteurised milk cheese was observed and the evolution of the volatile composition of the pasteurised cheese samples during ripening was clearly demonstrated. Partial least squares regression was used to examine possible relationships between the chemical-analytical and sensory descriptive data. r

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In order to explore the flavour produced by dark specialty malts, wort samples were brewed with different malts and evaluated by sensory and instrumental analysis. With increasing wort colour, a trained tasting panel detected more intense bitter and burnt flavours, whereas sweet and husky flavour notes decreased. Conversely, caramel and bread-like flavour attributes had a maximal intensity for the intermediate wort colours. Tasting of 20 EBC worts indicated that the flavour profile was significantly affected not only by malt level and malt colour but also by malt origin. Furthermore, the darkest caramel malt (480 EBC units) was found to contain most Maillard aldehydes as determined by the reaction with thiobarbituric acid. Similarly, other intermediate products of the Maillard reaction such as acetic acid, diacetyl and 2,3-pentanedione were found to arise in a higher concentration in dark caramel malts (220-480 EBC units) than in roasted malt (1200 EBC units). Dynamic headspace GC /MS further revealed that brewing with dark specialty malts considerably increased the level of 3-methylbutanal, its aldol condensation product (2-isopropyl-5-methyl-2-hexenal) and heterocyclic Maillard compounds. In contrast, dark malts drastically reduced the amount of hexanal in wort. By means of HPLC, it was established that only extreme roasting temperatures lead to the thermal degradation of ferulic acid to 4-vinylguaiacol in malt.

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