Background: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. Methods: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. Results: β-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. Conclusion: Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo.
Introduction: A fusion of the mandibular condyle with the base of the skull causes ankylosis of the temporomandibular joint (TMJ), which results in a lack of joint movement. The anesthesiologist is concerned about prolonged temporomandibular joint ankylosis because of the restricted airway, which makes intubation difficult. Despite the fact that TMJ ankylosis is a well-known risk factor for difficult airway management, the strategies used are based on the anesthesiologists' skill. It's never easy intubating a patient with temporomandibular joint ankylosis. The purpose of this study was to see how common problematic intubation was in TMJ ankylosis patients. Aim of the Study: The aim of the present study was to evaluate the incidence of difficult intubation in TMJ ankylosis patients. Materials and Methods: Among the overall data of patients attending Saveetha Dental College, a retrospective analysis of all the cases (patients who underwent TMJ ankylosis surgery) was extracted. Excel spreadsheets were used to enter the data. SPSS software version 19 was used to analyse the data collected. The data were statistically evaluated using the Chi square test. Results: In this study, it was discovered that the incidence of difficult intubation in TMJ ankylosis surgery was higher in the age group of 5-18 years than in the other age groups. Intubation in children is tough. The chi square test resulted in statistically significant findings (p<0.005). Conclusion: Nasal intubation and fiberoptic intubation were the most common modes of intubation used in TMJ ankylosis surgery, according to the results of this small sample retrospective analysis. The difficulty of intubation was increased in the paediatric age group (5-18 years).
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