The current investigation was to identify and characterise the rhizospheric isolates in coffee leaves (Coffea arabica L.) that have many functions for stimulating plant growth. Coffee leaves were harvested from the plants growing at the Lower Pulney Hills Regional Coffee Research Station in Thandigudi. Morphological and biochemical characterization of seven different rhizospheric bacterial isolates was conducted. Recent studies have found varying degrees of resistance in rhizospheric bacterial isolates. Nitrogen fixation, phosphate solubilization, and potassium mobilization are all characteristics that help plants thrive. Additionally, the rhizospheric bacteria were prospective plant symbionts for granting biotic and abiotic stress tolerance. The three effective isolates were subjected to 16S rRNA and 18S rRNA sequencing, and the findings revealed that CPR-2 was Bacillus subtilis, CPR-4 was Pseudomonas fluorescens, and CPR-7 was Trichoderma harzianum and CPR-8 was Rhizoctonia solani.
Coffee is an important plantation crop in India. Arabica and Robusta coffee are susceptible to fungal disease. Coffee plants propagated through seeds. Rhiozoctonia solani khun is known to cause collar rot/damping off of coffee seedlings in the nursery beds and lead to 10-25 % mortality of seedlings under favourable conditions. For effective management of this disease by bio capsule methods the present study was carried out, ten Rhiozoctonia solani khun isolates were collected from different regions. The pathogenicity effect to produce collar rot symptoms showed that R.s tn 4 is high virulent compared to other isolates during the period of April- June were noticed. In case of bio control agents such as Bacillus subtilis (58.65%) inhibition found to be maximum mean per cent inhibition compared to untreated control (70.50%)
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