We have developed a database of lymphoid polypeptides detected by two-dimensional polyacrylamide gel electrophoresis to aid in studies of leukemogenesis and of mutation affecting protein structure. In prior studies, we observed a 19-kDa phosphopolypeptide which was induced with proliferation in mature T cells and constitutively expressed in immature thymocytes. In this report we describe the identififcation of this polypeptide as the phosphorylated form of dUTPase (EC 3.6.1.23), following cDNA cloning of the gene, based on a partial amino acid sequence of the phosphopolypeptide. Studies of the expression and phosphorylation of dUTPase in human T cells indicate that accumulation and phosphorylation of dUTPase in mature T cells occur in a cell cycle-dependent manner. Interestingly, noncycling immature thymocytes express constitutively high levels of phosphorylated and unphosphorylated dUTPase. These results suggest an important role for dUTPase in immature thymocytes that is independent of proliferation.
1. After administration of 300 mg. of corticosterone or cortisol to a man, the greater part of these two corticosteroids were found in the urine in ester sulphate fraction. 2. Evidence that sulphate was attached to C-21 was provided by the similarity of chromatographic behaviour with that of authentic corticosterone 21-sulphate or cortisol 21-sulphate, electrophoretic separation for cortisol sulphate and the reaction to tetrazolium being positive only after hydrolysis. We are indebted to 'Expandia' and 'Societ6 Normande d'Extraction et de Synthese' and to 'Laboratoire Roussel' for the generous supplies of corticosterone and cortisol respectively.
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