Aim:Some literatures revealed that the high performance liquid chromatography (HPLC) method for single component or multicomponent analysis of montelukast sodium with other drugs. However, these methods deals with time consuming, so it is necessary to develop a cost-effective and less time consuming method for the estimation of doxofylline and montelukast sodium in combined pharmaceutical formulation.Materials and Methods:The separation was performed on an inertsil C8 (5 μm, 4.6 × 250 mm) column in isocratic mode with the mobile phase consisting a mixture of methanol and sodium phosphate buffer (75:25 v/v, pH 6.5 adjusted with orthophosphoric acid). The mobile phase was pumped at a flow rate of 1 mL min-1 and eluents were monitored at 230 nm.Results:The selected chromatographic conditions were found to separate doxofylline (retention time = 3.4 min) and montelukast sodium (retention time = 5.5 min) with a resolution of 5.47. The proposed HPLC method was validated with respect to linearity, accuracy, repeatability, specificity, robustness, and ruggedness as per International Conference on Harmonisation guidelines Q2(R1), November 2005 (Validation of Analytical Procedures: Text and Methodology). The percentage recoveries for doxofylline and montelukast sodium ranged from 98.1% to 101.7% and 98.2 to 101.9%, respectively, which indicated that the above method was enough accurate and precise.Conclusions:Hence, it was concluded that the developed method is suitable for routine analysis of these combination due to its less analysis time.
The observed experimental parameters such as density (ρ), viscosity (η) and ultrasonic velocity (U) of ternary liquid mixtures of primary alkanols such as 1-propanol, 1-butanol, 1-pentanol and 1-hexanol with N-N dimethylfomamide (DMF) in acetophenone at 303.15 K were determined. The observed experimental data have been utilized to evaluate some of the thermo acoustical parameters and also their excess values such as adiabatic compressibility (β E), intermolecular free length (L f E), free volume (V f E), internal pressure (π i E), Gibb's energy (∆G E) and viscosity (η E). Our present investigation observes that the interaction between DMF (Proton acceptor) and 1-alkanols is due to hydrogen bonding. There seems to be a dissociation of associated structure of 1-alkanols and decrease in interaction (hydrogen bonding) between acetophenone and 1-alkanols on further addition of DMF. It is also noticed that on further addition of DMF leads to subsequent decrease in interaction between ketone (acetophenone) and 1-alkanols as well as between DMF and 1-alkanol, which eventually resulting in existence of weak molecular interactions in the present system of liquid mixtures.
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