The Australian native plants Eucalyptus microcorys, Banksia serrata and Banksia oblongifolia were evaluated for their potential to produce tissue culture stocks under a simplified regime of growth substance treatment for shoot multiplication and root induction. The use of N 6 -benzyladenine (BA) alone proved to be successful with the three species in producing shoot multiplication, but only if shoot apices were used as explant material. Juvenile material provided best shoot proliferation, and by varying the concentration of BA, shoot elongation was manipulated. A combination of half-strength Murashige and Skoog (MS) medium and 0.25 J.lM BA proved to be the best compromise medium for shoot proliferation combined with shoot elongation. The use of indole-3-butyric acid (IBA) alone was successful in initiating roots from the three species. Root induction was easiest in E. microcorys, at an optimum concentration of 5 J.lM IBA, but the Banksia species were more difficult. The results demonstrate that a simplified protocol for shoot multiplication and root induction can be applied to a diverse group of Australian plants, which should result in more species being introduced into tissue culture programmes.