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In this study, heavy metal and microbial safety assessment of raw and cooked pumpkin and Amaranthus viridis leaves grown in Abakaliki, Nigeria, was examined. The levels of lead (Pb), arsenic (As), chromium (Cr), cadmium (Cd), and mercury (Hg) were evaluated using atomic absorption spectrophotometer. The microbial cells were counted and further identified to species level using 16S rDNA and ITS rDNA sequencing analysis at CABI microbial identification services United Kingdom (UK). The results showed that the heavy metal concentrations of lead (10.5, 12.0), arsenic (7.5, 8.5), chromium (0.9, 0.1), and mercury (13.1, 14.0) in the pumpkin and A. viridis leaves, respectively, were above maximum acceptable limit according to relevant national and international food regulatory agencies (Tables 1 and 2). Cooking significantlyreduced the concentrations of the heavy metals at (p > 0.05) to or below, lead (6.8, 8.4), arsenic (5.1, 6.1), chromium (0.6.0.1), and mercury (9.5, 11.4) in the pumpkin and A. viridis leaves, respectively, but still not to international safe limit. The result of the microbial safety assessment showed that the microbial load of both the pumpkin and Amaranthus viridis leaves were above acceptable limit and the contaminating organisms were identified as Escherichia coli (504743), Klebsiella pneumonia (504744b), and Aspergillus flavus (504740).This study therefore shows that the vegetables (pumpkin and A. viridis leaves) contain unacceptable levels of toxic heavy metals and potentially dangerous pathogenic microorganisms, thus present significant health risk for the consumers.

The chemical compositions, functional and pasting properties of flour from three varieties of Brachystegia spp. (Brachystegia eurycoma, Brachystegia nigerica and Brachystegia kennedy) were studied. Results show that the chemical compositions of flour samples ranged from12.31 to 12.67% (protein), 1.66 to 1.72% (crude fiber), 2.06 to 2.39% (ash), 7.18 to 8.45% (fat), 3.85 to 4.75% (sugar) and 58.45 to 59.62% (starch). Functional properties such as water absorption capacity, oil absorption capacity, swelling power, solubility index, pH and amylose content were in the ranges of 80.14 to 80.77, 84.21 to 84.52, 15.64 to 15.78, 15.44 to 15.98, 5.48 to 6.74 and 20.42 to 20.69%, respectively. In addition, pasting properties values were 85.58 to 89.05°C (peak temperature), 128.54 to 133.45 (peak viscosity), 23.75 to 26.53 (trough viscosity), 419.6 to 449.5 (final viscosity) 53.5 to 59.0 (break down viscosity) and 402.6 to 413.4 (Relative value units, RVU) (setback viscosity). No significant difference (p > 0.05) was observed in the functional properties of the flours. The pasting profile showed that peak and hot paste viscosities are the key pasting parameters in characterizing flours from the three Brachystegia varieties. The variation in peak viscosity of the Brachystegia flours might be due to varietals and geographical influence. The study shows that B. eurycoma flour had the best functional and pasting properties results that could be exploited in food formulations such as soup, and sauces.

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The proximate and microbiological compositions of kilishi, a processed meat at Abakaliki, southeast of Nigeria was determined in this study. The moisture, protein, fat, and ash contents, including the microbial count of the raw meat and kilishi in the study area were investigated to ascertain the processing effect. The results show that kilishi has higher protein content, fat content and ash content relative to the raw meat with the difference of 17.04 %, 4.66 % and 1.94 %. The moisture content and microbial count of kilishi are lower compared to the raw meat with a difference of 62.00 % and 17.33 cfu/g. The analysis suggests that the kilishi sold at Abakaliki is safe for human consumption.

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