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Exposures to airborne metals are known to cause physiological responses in organisms and wide-ranging health effects in humans. Hence determination of metals in particulate matter is important from a toxicological perspective. In the current study heavy metals associated with respirable (RSPM) and nonrespirable (NRSPM) fractions of suspended particulate matter were estimated in air samples from six stations in Coimbatore, India, during March 1999 to February 2001. The mean quantity of heavy metals in RSPM was in the order Zn > Cu > Pb > Ni > Cr > Cd. Concentrations of these heavy metals were in the range of BDL (below detectable level) to 2,147 ng/m3 in RSPM. The highest level of lead (2,147 ng/m(3)) was recorded at an industrial station. The station also had the highest mean value (481 +/- 544.3 ng/m(3)), suggesting the importance of industrial operations in determining the ambient concentrations of lead. Significant positive correlation among metals excepting lead and copper suggests that they originate mostly from a common source. Air samples of urban and industrial areas showed higher concentrations than residential (Urban) and suburban areas.

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Measurements of morphological features are important for ecological studies, especially on free‐ranging wild animal species. Conventionally, specimens either dead or in captivity are used for morphometric studies, which is difficult in the case of wild species for several reasons. Capturing would be even futile when research questions are relating to issues such as prey size selection or estimation of intake rate under field conditions, where in situ morphometric measurements are inevitable. Remotely estimating morphometric features via, avian bill and head lengths are always laden with wide variances. Hence, we developed this technique for in situ measurements of animal morphology through digital photographs. This non‐invasive novel method for in situ estimation of individuals’ morphometric measurements uses digital photographs’ metadata. We extracted the metadata (field of view, focal length, subject‐distance, etc.,) in exchangeable image file format from digital images using freely available software, and the unknown sizes were estimated from each image. Cardboard (fish) models were, at first, used to estimate the accuracy of measurements comparing actual and estimated values, and the same protocol was repeated on museum specimens (bird). Finally, we checked the repeatability of this method under field conditions using free‐ranging Chickens (Gallus gallus domesticus). The results under different conditions were subjected to rigorous statistical testing. Upon comparing the actual with estimated values, obtained from three distinct types of objects (1. Cardboard model, 2. Museum specimens and 3. Live birds under field conditions), variations were found non‐significant affirming the assumption that actual value (Y) = estimated value (X) with inconsequential variance of the Y from X. Moreover, both the relative and absolute errors between the actual and estimated values were so low, indicating that this method yielded neither overestimation nor underestimation. The advantage of this non‐invasive method is that it could generate reasonably accurate morphometric measurements at a relatively low cost for routine morphometric works in field conditions. We envisage significant utility of this non‐invasive technique in different fields of science such as biology, morphology, ecology, evolution, wildlife, conservation science, agriculture, forensics and engineering design.

We measured the background level and spatial variation of carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) and associated basic soil parameters along the depth profile for 3 years, in 3 habitats (woodland, wetland, and grassland), in Keoladeo National Park (KNP), an important water fowl reserve and a world heritage site in India. The study examined soils at 5 depths (0, 0.25, 0.50, 0.75 and 1.0 m) for 3 years, i.e. from 2003 to 2005. Total organic C (TOC), total N (TN), total available P (TAP), and total available S (TAS), irrespective of the habitat type and year, were found to be highest in the litter layer, gradually declining with depth. Elemental ratios (C : N, C : P, and C : S) followed the same declining trend, whereas the N : P ratio increased down the soil profile. The high C : N and C : S ratios in the litter layer suggest the relatively low mobilisation of N and S from the decaying detritus layer. All the variables studied varied significantly among the soil layers (GLM-ANOVA, P < 0.05). Principal component analysis (PCA) showed 4 components based on examination of the scree plot. The first component accounted for 27.1% of the total variance in soil characteristics among samples, reflecting the influence of soil variables such as P, TOC, and pH. The second component accounted for 23.5% of the total variance, reflecting the influence of total dissolved solids (TDS) and TAS. The influence of C on elemental ratio (C : S) was pointed by the third component, accounted for 14.2% of the total variance. The fourth component accounted for 13.6% of the variance, indicating the influence of soil TN. Thus, the 4 PCA components that accounted for 78.4% of the total variance in the data can be qualified as N : P/soil P/C, TDS/TAS, C : S ratio, and soil TN, respectively.

It has been confirmed that mammalian sperm contain thousands of functional RNAs, and some of them have vital roles in fertilization and early embryonic development. Therefore, we attempted to characterize transcriptome of the sperm of fertile chickens using microarray analysis. Spermatozoal RNA was pooled from 10 fertile males and used for RNA preparation. Prior to performing the microarray, RNA quality was assessed using a bioanalyzer, and gDNA and somatic cell RNA contamination was assessed by CD4 and PTPRC gene amplification. The chicken sperm transcriptome was cross-examined by analysing sperm and testes RNA on a 4 × 44K chicken array, and results were verified by RT-PCR. Microarray analysis identified 21,639 predominantly nuclear-encoded transcripts in chicken sperm. The majority (66.55%) of the sperm transcripts were shared with the testes, while surprisingly, 33.45% transcripts were detected (raw signal intensity greater than 50) only in the sperm and not in the testes. The greatest proportion of up-regulated transcripts were responsible for signal transduction (63.20%) followed by embryonic development (56.76%) and cell structure (56.25%). Of the 20 most abundant transcripts, 18 remain uncharacterized, whereas the least abundant genes were mostly associated with the ribosome. These findings lay a foundation for more detailed investigations on sperm RNAs in chickens to identify sperm-based biomarkers for fertility.

BackgroundThe present study is an attempt to explore the association between kitchen indoor air pollutants and physiological profiles in kitchen workers with microalbuminuria (MAU) in north India (Lucknow) and south India (Coimbatore).MethodsThe subjects comprised 145 control subjects, 233 kitchen workers from north India and 186 kitchen workers from south India. Information related to the personal and occupational history and health of the subjects at both locations were collected using a custom-made questionnaire. Worker lung function was measured using a spirometer. Blood pressure was monitored using a sphygmomanometer. Urinary MAU was measured using a urine analyzer. Indoor air monitoring in kitchens for particulate matter (PM), total volatile organic compounds (TVOC), carbon dioxide (CO2) and carbon monoxide (CO) was conducted using indoor air quality monitors. The size and shape of PM in indoor air was assessed using a scanning electron microscope (SEM). Fourier transform infrared (FTIR) spectroscopy was used to detect organic or inorganic compounds in the air samples.ResultsParticulate matter concentrations (PM2.5 and PM1) were significantly higher in both north and south Indian kitchens than in non-kitchen areas. The concentrations of TVOC, CO and CO2 were higher in the kitchens of north and south India than in the control locations (non-kitchen areas). Coarse, fine and ultrafine particles and several elements were also detected in kitchens in both locations by SEM and elemental analysis. The FTIR spectra of kitchen indoor air at both locations show the presence of organic chemicals. Significant declines in systolic blood pressure and lung function were observed in the kitchen workers with MAU at both locations compared to those of the control subjects. A higher prevalence of obstruction cases with MAU was observed among the workers in the southern region than in the controls (p < 0.01).ConclusionsKitchen workers in south India have lower lung capacities and a greater risk of obstructive and restrictive abnormalities than their north Indian counterparts. The study showed that occupational exposure to multiple kitchen indoor air pollutants (ultrafine particles, PM2.5, PM1, TVOC, CO, CO2) and FTIR-derived compounds can be associated with a decline in lung function (restrictive and obstructive patterns) in kitchen workers with microalbuminuria. Further studies in different geographical locations in India among kitchen workers on a wider scale are required to validate the present findings.

Bisphenol A (BPA) acts as an endocrine disruptor, affects animal reproductive success in vivo and affects sperm functions in vitro at environmentally relevant concentrations, leading to reduction in sperm motility and fertilizing ability in fish. The effect of in vitro BPA on avian sperm functions has not been explored. The present study examined the effect of environmentally relevant concentrations of BPA (0 mM, 0.18 mM, 0.37 mM, and 0.74 mM) on sperm functions in chicken in vitro. Sperm were exposed to concentrations of BPA for 30 min and analyzed for motility, fertilizing ability, live sperm percentage, and mitochondrial membrane potential (Δψm). Results showed that BPA at a concentration of 0.74 mM significantly decreased motility, fertilizing ability, live sperm count percentage, and sperm Δψm. Sperm motility was positively correlated with fertility (r = 0.73, p ≤ 0.01), live sperm percentage (r = 0.64, p ≤ 0.01), and high Δψm (r = 0.44, p ≤ 0.01). A dose-dependent and time-dependent effect of BPA was observed on sperm motility at all BPA concentrations. However, sperm's fertilizing ability was unaffected in low BPA concentration (0.18 mM and 0.37 mM). A significantly higher percentage of moribund sperm was observed at 0.37 mM and 0.74 mM BPA compared with at 0.18 mM BPA, in the negative control, and in the vehicle control. The present study confirms that environmentally relevant concentrations of BPA are capable of compromising sperm functions, leading to reduction in fertilizing ability of chicken sperm.