In the study, silver nanoparticles (AgNPs) were successfully synthesized by an environmentally friendly synthesis method using Seseli resinosum Freyn & Sint extract. The synthesized silver nanoparticles were characterized by ultraviolet/visible light absorption spectrophotometer (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analysis. As a result of the characterization, it was determined that 33 nm spherical nanoparticles were formed, showing a spectrum at 420 nm wavelength. Silver nanoparticles showed a bacteriocidal effect against all bacterial strains. DPPH and ABTS methods were used to examine the antioxidant activities of plant extracts and AgNPs. In DPPH removal activity, AgNPs obtained by green synthesis provided a high rate of inhibition removal compared to the extract. According to this percentage, while silver nanoparticles provided 22% removal, the extract provided 15% removal. In ABTS removal activity, when AgNPs were obtained by green synthesis compared to the extract, silver nanoparticles provided 25% removal, while the extract provided 18% removal. The characterization of silver nanoparticles synthesized by the green synthesis method and their antioxidant activity were investigated, and the obtained values indicate the presence of an antioxidant capacity. In addition, the inhibitory effects of the extract and AgNP on lipoxygenase activity, which has an important place in health, were investigated. It was determined that the aqueous extract of S. resinosum and the AgNP synthesized from the extract had lipoxygenase enzyme inhibitory activity.
In this study, water and methanol extracts were obtained from sea buckthorn seeds, which have an important place in health. It was aimed to determine the DPPH, ABTS antioxidant activities and urease, collagenase enzyme inhibition activities of these extracts. The inhibitory effect of the prepared extracts on these enzymes was evaluated by in vitro method. The best DPPH and ABTS scavenging activity was observed in aqueous extracts. DPPH (IC50 = 19.34±0.49 µg/mL) and ABTS (IC50 = 7.24±0.14 µg/mL) were determined. According to DPPH results, while aqueous extract provided 25,85 % removal, the methanolic extract provided 20,44 % removal. In ABTS removal activity, while aqueous extract provided 25,55 % removal, the methanolic extract provided 18,23% removal. The lower the IC50 value, the higher the free radical scavenging power. The best urease and collagenase inhibition from sea buckthorn seed extracts was seen in the aqueous extract (IC50 = 0,23 ± 0,003μg/mL, IC50 = 2,19 ± 1,12μg/mL). Methanol extracts did not show tyrosinase and urease enzyme inhibition. This study clearly shows that sea buckthorn the aqueous extract of can be used as an alternative source of anti-urease and anti-collagenase source. Due to the important role of these enzymes in various diseases, new drugs with inducing or inhibitory effects should be developed.
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