The purpose of this study was to investigate the effects of filling and lining materials in various compositions on Streptococcus mutans. Five glass-ionomer cements (Vitrebond, XR Ionomer, Ketac-Bond, Shofu Base Cement, Shofu Lining Cement) and two composite materials (Heliomolar-RO, Concise) were used in this study. Samples from these materials were prepared in sterilized conditions and hung in tryptic soy broth medium containing 5% sucrose. The media were inoculated with Streptococcus mutans ATCC 13419. After 5 days.the plaque that had accumulated on the surfaces was scraped off and weighed in wet and dry conditions. Then the colonies were counted and evaluated in comparison with each other. The least amount of plaque and colony numbers were found on Vitrebond material disks. In conclusion, there were no differences among XR Ionomer, Heliomolar-RO and Ketac Bond. Concise gave better results than some materials containing fluoride; whereas the most abundant amounts of plaque were found on Shofu Lining Cement and Shofu Base Cement. Introduction ,.
Objective: Toothbrushes may become contaminated after a single use by a wide array of microorganisms which are present both in the oral cavity and in the external environment. The retention and survival of microorganisms may depend on brush design and toothpaste usage. We aimed to investigate the effect of the brush tuft design and the use of toothpaste on the cariogenic microbial contamination of toothbrushes.Method: Totally 11 dental students whose salivary mutans streptococci levels were >10 6 cfu/mL, brushed their teeth using toothbrushes with two different tuft design using once at one-week intervals. The experiment was also repeated using a triclosancontaining toothpaste. After use, each toothbrush was rinsed under running tap water for 10 s and four tufts were processed for total bacteria, mutans streptococci, lactobacilli and yeast counts using culturing techniques.Microbial colony-forming units for a given brush type for the same volunteers were compared using Wilcoxon test. Results:No statistically significance was found in terms of mutans streptococci, lactobacilli, yeast and total bacteria adhesion between the two types of toothbrushes (p>0.05). Brushing with toothpaste,
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