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Objectives: Dental anxiety is a significant determinant of regular dental visits. The objectives of the study were to determine the prevalence and determinants of dental anxiety among adult patients at the dental center of the University of Benin Teaching Hospital. Subjects and Methods: A total of 390 respondents aged 16-89-year-old were interviewed for the study. The Participants were selected through a predetermined sequence of systematic random sampling and completed a questionnaire based on the Coral Dental Anxiety Scale (CDAS). Data were analyzed using Statistical Package for Social Sciences version 20.0 (SPSS version 20, IBM Statistics, New York, USA) and means compared using Student's t-test and analysis of variance. Results: The prevalence of dental anxiety (CDAS ≥ 13) in the study population was 8.7% (n = 34), and the overall mean and standard deviation of CDAS was 7.81 ± 2.50. The total mean dental anxiety score and mean for each item were significantly higher in females than in males (t = 6.17 P = 0.016). There was a significant decrease in mean CDAS score with age (F = 14.51, P = 0.005). Mean CDAS score was significantly higher in respondents without formal education than those with formal education (t = 7.41, P = 0.005). The mean CDAS scores for root canal therapy were 9. 66 ± 3.16; extraction, 8.84 ± 2.33; filling, 6.45 ± 2.81; and scaling, 5.12 ± 1.87. The highest and lowest means were not significantly different (t = 2.73, P = 0.07). Conclusion: The prevalence of dental anxiety in the study sample was low. Whereas dental anxiety was significantly associated with age, gender, and educational status, there was no significant relationship between dental anxiety and type of dental treatment.