Ageing-induced increase in inflammatory gene expression through a reduction in DNA methylation might contribute to chronic diseases. Regular physical exercise practices, in turn, are associated with a decrease in the incidence of inflammatory diseases. We herein evaluated the effects of three exercise modalities on lymphocyte global and gene-specific (interferon γ (IFN-γ) and interleukin 17A (IL-17A) DNA methylation in aged women (68 ± 7.5 years). This cross-sectional study included 86 women, divided into four groups according to the physical exercise practice: 20 were practicing resistance training (RT); 24 were practicing water aerobics exercise (W); 22 were practicing water aerobics and resistance exercise (RWT), and 20 did not practice any physical exercise (CON). We evaluated volunteer functional capability using the Timed Up and Go (TUG) test, global lymphocyte DNA methylation by enzyme-linked immunosorbent assay, IFN-γ and IL-17A methylation by qPCR and CD4 + IFN-γ + and CD4 + IL-17 + cell percentage by flow cytometry. The three physically exercised groups performed functional capability tests in a shorter period and showed a higher global lymphocyte DNA methylation and methylated CpGs of IL-17A and IFNγ promoter regions than the control group. The practice of resistance training (RT and RWT groups) lead to high global DNA methylation. The combination of resistance training and aerobic exercise led to the increase of lymphocyte IL-17A and IFN-γ gene methylation induced by each separately. However, the percentage of IFN-γ +
Introdução: A suplementação de aminoácidos de cadeia ramificada (BCAA) tem sido amplamente utilizada e estudada durante as atividades de longa duração, no entanto, apresenta um número restrito de estudos elucidando o efeito desta suplementação durante o treinamento resistido (TR). Objetivo: Avaliar a influência da ingestão de BCAA, contendo diferentes concentrações de leucina, sobre o número total de repetições realizadas durante essa prática. Métodos: Oito participantes (4 homens e 4 mulheres; idade: 20 a 35 anos), com experiência no TR entre 3 e 18 meses, realizaram 10 séries com carga referente a 80% de 1RM, após a ingestão de 0,4 g/kg de peso corporal de BCAA diluídos em 250 ml água, com duas diferentes concentrações de leucina: 4:1:1 (3 g leucina, 750 mg isoleucina e 750 mg valina) e 6:1:1 (4,5 g leucina, 750 mg isoleucina e 750 mg valina), além do tratamento controle (CON), caracterizado pela ingestão de 250 ml água contendo um composto dietético não calórico. Resultados: Observou-se que o número total de repetições realizadas nas 10 séries foi significativamente maior para o tratamento 6:1:1 (70,0 ± 9,5) quando comparado ao tratamento CON (59,8 ± 9,9), diferente do tratamento 4:1:1 (66,8 ± 7,3) que apresentou resultado estatisticamente semelhante. Conclusão: A concentração do aminoácido leucina, relacionada à dose de BCAA suplementada, é significativa para obtenção dos efeitos ergogênicos promovidos por este suplemento durante a prática do exercício resistido.
Although any type or amount of physical exercise may reverse the cellular processes that lead to the aging, regular physical exercise can attenuate the decline in physical and functional abilities observed in elderly. Currently, the most encouraged activities in gyms and clubs are resistance training, functional training, and hydrogymnastics. The main objective of this study was to compare the effects of the aforementioned exercises regarding muscle strength, flexibility, functional capability and quality of life of elderly women. We divided 36 elderly women in four groups: Sedentary (S); Hydro-gymnastics (H); Functional Training (F); or Resistance Training (RT). The results reveal that the groups engaged in physical exercise showed a significant difference (p < 0.05) in relation to S in all evaluations. The results also show that between the different exercises evaluated, resistance training practitioners have greater strength and better functional capability.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.