Atmospheric Particulate Matter (PM) is considered one of the most important air pollutants in terms of its health impacts, environmental degradations and visibility. Particles size, their chemical composition and atmospheric level are important factors for determining their adverse health impacts. In this paper the main objective of this work was focus on PM 10 and its bio-accumulations (microbial load) in Makkah during Hajj seasons 2016 (1437AH). This study concluded that the concentration of respirable particles (PM 10 ) and its watersoluble ions were much higher at site 2, site 1 than that at sites 3, 4 and 5 around Haram in Makkah. In addition, Man is the main source of air microbial contamination surrounding Haram in Makkah. Fungi evidence of the lack of output from natural sources of pollution. Spotted Staphylococcus bacteria were at high rates in all sites and micro-organisms at the site 2. The source of microbial contamination in all sites was the same, while the source of fungi differs especially at the site 2. The natural resources were the most predominant at the site of 2 and 1, as compared to sites 3, 4 and 5.