Background: Cervico-vaginal infection is one of the most common problems in clinical medicine. This study aimed to the most common causes of cervical-vaginal infections among Sudanese women complaining of vaginal discharge. Method: Cross-sectional hospital based study that had carried out on one hundred non-pregnant women complaining of vaginal discharge attended at gynecology department -Khartoum teaching hospital. All subjects had signed informed consent. Smear from all collected samples were prepared and stained with Papanicolaou Stain for cytological evaluation. Descriptive and analytical statistics (chi -square test) were applied using SPSS (Version 20). Results:The result showed that the majority of subjects were from central and northern of Sudan. The mean age of women was (29) The most common microorganisms seen were bacilli bacteria (49%), followed by candida (18%), Actinomycesisraelii, Leptothrix spp. and Gardnerellavaginalis were (2%), (2%), and (1%) respectively. Statistically no significant association between infection and age group, circumcision, geographical location, and occupation, p value of all these variables was above than significant level 0.05. Conclusion:The most prevalent infections were bacterial vaginosis and candida albicans. This is consistent with the results of many studies although other studies reported different results.