No abstract

No abstract

Abstract:The hypoglycaemic potentials of frog oil in alloxan-induced diabetic rats were investigated. Thirty six male albino rats weighing 120.47±2.52 g was completely randomized into six groups (A-F) comprising 6 animals each. Animals in group A (control) were administered 1 ml of distilled water while those in groups B, C, D, E and F were induced with diabetes mellitus by intraperitoneal administration of alloxan monohydrate (180mg/kg body weight) and in addition were respectively administered distilled water, metformin (a reference antidiabetic drug), 3, 6 and 9 mg/kg body weight of frog oil once daily. Treatment with the oil lasted for 14 days during which blood glucose level and selected biochemical parameters were determined. The results showed that there was significant (p<0.05) reduction in glucose levels in the group treated with 9.0 mg/kg body weight of the oil from 221.22±0.15 to 100.15±0.07 mg/dl, indicating the best antidiabetic activity of all the treatment groups. The oil also caused significant (p<0.05) decrease in serum total bilirubin levels from 8.73±0.07 µmol/L to 2.43±0.03 µmol/L; serum total cholesterol levels from 313.48±0.05 mmol/L to 232.40±0.19 mmol/L; liver aspartate aminotransferase (AST) activity from 76.93±0.02 U/L to 35.25±0.02 U/L; liver alanine aminotransferase (ALT) activity from 85.52±0.05 U/L to 39.71±0.08 U/L respectively. Overall, these findings established the fact that frog oil has hypoglycaemic potentials and thus can be recommended for use in the treatment of diabetes. The results from biochemical parameters indicated that frog oil could also be explored in the control of some of the metabolic dysfunctions normally associated with diabetes.

A knowledge of the chemical content of Rhinoceros (Rhino) oil and its activity on selected biomolecules of experimental models would help boost the immune system against an immunocompromised COVID-19 status. The study seeks to evaluate the chemical and biomolecular profile of Rhino oil. Chemical profile was done using standard methods of analysis. 25 rats were assigned in five groups (A-E) (n=5). Animals in group A (control) were administered 0.5 ml of distilled water while those in groups B-E which were immunocompromised (by intraperitoneal administration of 5 mg/kg body weight (b.w) of lead [Pb]) were also administered distilled water, immunomodulatory drug (5 mg/kg body weight of zinc [Zn]), 2 and 5 mg/kg b.w of Rhino oil extract respectively, once daily for 8 days followed by biomolecular assay. Proximate analysis gave moisture content (14.37±0.29), among others. FAMEs analysis showed hexadecanoic acid (12.80%) and other esters. Lipid profile of the oil gave LDLC to contain (32.90±0.53 mg/L), and others. The physicochemical properties gave iodine value as (115.80±0.40 mg/g), among others. The metal composition revealed Zn (0.28±0.06) plus others. The amnio acid profile of the oil gave ten essential amino acids and non-essential amino acids respectively. The levels of biomolecules in serum of the animals were altered at specific doses of the oil extract. Altogether, the chemical content of the oil was significantly high, with altered biomolecular effect. The rich content of vital nutrients and chemicals of Rhino oil may boost the white blood cells against COVID-19. The isolation and characterization of the active principles of the oil is encouraged.

No abstract