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The objective of this study was to evaluate the effects of defoliation frequency (either at two-or three-leaf stage) and nitrogen (N) application rate (0, 75, 150, 300, 450 kg N ha À1 year À1 ) on herbage carbohydrate and crude protein (CP) fractions, and the water-soluble carbohydrate-to-protein ratio (WSC:CP) in perennial ryegrass swards. Crude protein fractions were analysed according to the Cornell carbohydrate and protein system. Carbohydrate fractions were analysed by ultra-high-performance liquid chromatography. Sward defoliation at two-leaf stage increased the total CP, reduced the buffer-soluble CP fractions and decreased carbohydrate fractions of herbage (P < 0Á001). The effect of defoliation frequency was less marked during early spring and autumn (P < 0Á001) than for the rest of the seasons. An increase in N application rate was negatively associated with WSC, fructans and neutral detergent fibre (P < 0Á001), and positively associated with CP and nitrate (N-NO 3 ) contents of herbage. Nitrogen application rate did not affect CP fractions of herbage (P > 0Á05). The fluctuations in CP and WSC contents of herbage resulted in lower WSC:CP ratios during early spring and autumn (0Á45:1 and 0Á75:1 respectively) than in late spring (1Á11:1). The herbage WSC: CP ratio was greater (P < 0Á001) at the three-leaf than the two-leaf defoliation stage and declined as the N application increased in all seasons (P < 0Á001). The results of this study indicate that CP and carbohydrate fractions of herbage can be manipulated by sward defoliation frequency and N application rate. The magnitude of these effects, however, may vary with the season.

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The objective of this study was to determine, under the soil and climatic conditions of Southern Chile, the effect of the ploidy of perennial ryegrass (Lolium perenne L.) cultivars on herbage production, nutritive value, grazing preference and utilization of pasture produced. This study was conducted in southern Chile, Valdivia Province, and was evaluated for 3 years. The tetraploid cultivars used were Quartet (4n), Gwendal (4n), Pastoral (4n) and Napoleon (4n). The diploid cultivars were Anita (2n), Jumbo (2n), Aries (2n), and Yatsyn 1 (2n). When the average sward height reached 20 cm, all plots were simultaneously grazed by dairy cows for a period of 24 h. Before and after grazing, sward height, dry matter availability and nutritive value were evaluated. Grazing preference was visually assessed every 5 min for a period of 2.5 h after the afternoon milking. During the 3-year period 20 grazing events were evaluated. A randomized complete block design, with eight cultivars and three replicates, was used. Diploid cultivars showed greater herbage mass accumulation than tetraploid cultivars (P ≤ 0.05). No significant differences were obtained in the annual average crude protein content. Nevertheless, tetraploid cultivars showed a greater D value than diploid cultivars, except during the third year when the difference was not statistically significant. Dairy cows grazed more time on tetraploid cultivars. Considering, additionally, the residual herbage mass after grazing and the percentage of pasture utilization, diploid cultivars were less intensively grazed, suggesting a lower consumption by the cows.

A mini-sward study was undertaken between January and September 2008 in Valdivia, Chile, to quantify and compare changes in the herbage production, growth dynamics and nutritive quality of a Lolium perenne sward during autumn in response to different nitrogen (N) application regimes and defoliation frequencies. The N levels used in the present study corresponded to the equivalent of an autumnal application of 0, 25 or 75 kg N/ha. The defoliation frequency was based on leaf regrowth stage, and corresponded to the time taken for 1.5, 2.5 or 3.5 leaves per tiller to expand. The growth dynamic measurements were also related to temperature. The results showed that the level of N had no effect on the studied variables. Plants defoliated at the 3.5-leaf stage showed a higher herbage production, higher tillering and a more balanced nutritional profile than plants cut at the 1.5-leaf stage. The defoliation interval had no effect on leaf elongation or leaf appearance. The leaf elongation rate was linearly related to the mean daily temperature (P < 0.01), and the accumulated leaf and tiller number showed a significant linear relationship (P < 0.01) with the thermal time expressed as growing degree days. From the defoliation frequencies tested here, we can conclude that the best time for grazing L. perenne swards during autumn is at the 3.5-leaf stage due to a higher herbage production, higher tillering rate, more balanced nutritive quality and higher reserve levels of the plant, which provide a more favourable background for subsequent growth.

Prairies are a main source for livestock feeding in southern Chile. The aim of this research was to define how grazing events and natural wetting and drying cycles (WD) affect the spatial and temporal variability of the soil's structural properties. The investigation was conducted in a Duric Hapludand, Valdivia Series. Penetration resistance (PR) and volumetric water content (WC), measured in situ, were used to prepare maps which show i) temporal (1383 to 3047 kPa for 46 to 16% WC) and spatial changes, and ii) grazing events as an important factor influencing spatial changes in PR (differences of 3421 kPa between max and min values). Grazing and WD cycles induced changes in the soil's mechanical stability and pore functions, which indicate that structure-dependent properties are dynamic. During the study, variations between 0.3 and 0.9 log µm 2 were detected for air permeability (ka), whereas air capacity (ACp) ranged between 5 and 18%. Soil mechanical strength also varied over time and showed changes in PR. The same instrument, however, cannot be used to identify changes in soil pore functions. Generally, after grazing events, soil deformation induced a reduction of air capacity and permeability; however, after WD cycles, soil pores were able to recover their functional integrity.

The objective was to evaluate water‐soluble carbohydrate (WSC) and crude protein (CP) concentration of perennial ryegrass (PRG) cultivars with different genetic potential for producing WSC under two contrasting agronomic managements in temperate climate (southern Chile). A 4 × 2 factorial design was randomly allocated to 24 plots (31 m2 each, three blocks): four PRG cultivars (diploid standard cultivar, “2nSt”; tetraploid standard cultivar, “4nSt”; diploid high sugar cultivar developed in New Zealand, “2nHSNZ”; and tetraploid high sugar cultivar developed in Europe, “4nHSEU”) and two agronomic managements (“favourable,” defoliations at three leaves per tiller and nitrogen (N) fertilization rate of 83.3 kg N ha−1 year−1; “unfavourable,” defoliations at two leaves per tiller and N fertilization rate of 250 kg N ha−1 year−1). Herbage samples were collected in early spring, spring, summer and autumn. Concentration of WSC did not differ among cultivars in spring and summer, averaging 194 and 251 g/kg DM, respectively. The cultivar 4nHSEU had the greatest WSC concentration in early spring and autumn (187 and 266 g/kg DM, respectively) and the greatest CP concentration across samplings (average 230 g/kg DM). Favourable management improved WSC concentrations in early spring and summer and decreased CP in spring, summer and autumn. Annual DM yield did not vary with cultivar or management, averaging 8.43 t/ha. Within a 12‐month study at one site in a temperate environment in southern Chile, PRG cultivars have not shown a consistent expression of the “high sugar” trait, where a genetic × environment interaction might be operating.