Aim of the study Bone morphogenic proteins (BMPs) have both inhibitory and stimulatory effects on growth of a tumor that depend on the type of cells, the dosage and the tumor microenvironment. We aimed to investigate the impact of the bone morphogenic protein-7 (BMP-7) single nucleotide polymorphism (SNP) rs230205 [A/G] on susceptibility to hepatocellular carcinoma (HCC) progression from liver cirrhosis after viral hepatitis infection in Egyptian patients. Material and methods The amplification-refractory mutation system (ARMS)-polymerase chain reaction (PCR) method was used to genotype the rs230205 [A/G] SNP in 150 subjects (50 patients with post-hepatitis C or B cirrhosis, 50 HCC patients, and 50 controls). Expression level of BMP-7 protein was assessed using enzyme-linked immunosorbent assay (ELISA). Results The results revealed insignificant changes in distribution of all genotypes/alleles of the BMP-7 rs230205 [A/G] SNP between cirrhotic patients, HCC patients and controls. The AA genotype and A allele could be considered risk factors for cirrhosis (OR = 1.75, 1.50) and HCC (OR = 2.19, 1.74), respectively. The AA genotype (95% CI: 0.45-6.79) and A allele (OR = 1.50, 95% CI: 0.77-2.93) may be viewed as cirrhosis risk factors based on group segregation. Additionally, the A allele, AG and AA genotypes and their combined ORs of 2.19 (95% CI: 0.58-8.23), 1.74 (95% CI: 0.90-3.37), and 1.70 (95% CI: 0.68-4.29) could all be risk factors for HCC. No genotype or allele could be regarded as a risk factor for progression of cirrhosis to HCC, according to OR values. Conclusions The results showed no correlation between BMP-7 rs230205 [A/G] SNP and progression of cirrhosis to HCC. To confirm our findings, additional prospective large-scale research is required.
The bone morphogenic protein-7 (BMP-7) gene is a member of the transforming growth factor-β (TGF-β) superfamily. BMP-7 is believed to act as a regulator of endogenous control of hepatocytes proliferation and liver homeostasis in adult hepatocytes. The present study aimed to assess the association of single nucleotide polymorphism (SNP), rs162316 (G>A), in BMP-7 gene with the progression of HCV or HBV-chronic liver cirrhosis to hepatocellular carcinoma (HCC). The tetra-primer amplification-refractory mutation system (ARMS-PCR) method was used for rs162316 genotyping. In this study, 150 subjects were divided into 50 healthy controls, 50 HCV or HBV-cirrhotic patients with HCC and 50 HCV or HBV-cirrhotic patients and HCC-free. Results of rs162316 genotyping revealed an association of SNP with 1.14 (0.64-2.03) and1.43(0.79-2.58) alleles A frequencies (OR, CI 95%) in HCC patients compared to cirrhotic and healthy control groups respectively. For the specific studied SNP of BMP7 gene rs162316, the A allele (AG genotype) and was significantly associated with HCC progression. However, AG genotype of rs162316 may be considered as a predictor for HCC in Egyptian cirrhosis patients. (2013): Prognostic significance of BMP7 as an oncogene in hepatocellular carcinoma. Tumor Biol.; 34:669-674.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.