Progesterone receptor (PGR) is indispensable for pregnancy in mammals. Uterine PGR responds to the heightened levels of ovarian progesterone (P4) after ovulation and regulates uterine gene transcription for successful embryo implantation. Although epithelial and stromal P4-PGR signaling may interact with each other to form appropriate endometrial milieu for uterine receptivity and the subsequent embryo attachment, it remains unclear what the specific roles of epithelial P4-PGR signaling in the adult uterus are. Here we generated mice with epithelial deletion of Pgr in the adult uterus (Pgrfl/flLtfCre/+ mice) by crossing Pgr-floxed and Ltf-Cre mice. Pgrfl/flLtfCre/+ mice are infertile due to the impairment of embryo attachment. Pgrfl/flLtfCre/+ uteri did not exhibit epithelial growth arrest, suggesting compromised uterine receptivity. Both epithelial and stromal expressions of P4-responsive genes decreased in Pgrfl/flLtfCre/+ mice during the peri-implantation period, indicating that epithelial Pgr deletion affects not only epithelial but stromal P4 responsiveness. In addition, uterine LIF, an inducer of embryo attachment, was decreased in Pgrfl/flLtfCre/+ mice. The RNA-seq analysis using luminal epithelial specimens dissected out by laser capture microdissection revealed that the signaling pathways related to extracellular matrix, cell adhesion and cell proliferation are altered in Pgrfl/flLtfCre/+ mice. These findings suggest that epithelial PGR controls both epithelial and stromal P4 responsiveness and epithelial cell differentiation, which provides normal uterine receptivity and subsequent embryo attachment.
Low level laser has been conducted for treatment of bone fracture on animal and had shown positive results. If laser energy is delivered at optimal dose with appropriate parameters, it might get best results however a little research in the literature has conducted on the effect of low level laser on human bone fracture. The aim of the study was to assess the radiological and clinical difference of the effect of low level laser therapy on the delayed bone healing of patients with different bone fractures. This was a prospective study conducted in Benha university hospital on 33 patients with delayed and non-union of bone fractures via low level laser diode laser with a wavelength 905nm. There were 27 male 5 female patients, mean age group ±34.43 range (24 years to 67 years), the mean delayed of union was ± 6,68 range (3 months to 15 months ). At the end of the study, union is achieved in mean period±1,7( 1 month to 4,1 month)which led to success of about 70% improvement of the treated group, union mean number of sessions used ±23,375 (range 15 sessions to 50 sessions), Mean Visual Analog Scale (VAS) ± 1,3 range from (0 to 6). the present study indicate that Low level laser therapy is an effective modality for treatment of delayed bone fracture, can relieve pain and increase range of motion of the associated stiff joints with no adverse effect provided that adequate patient selection has conducted.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.