Abstract. Expression of receptor tyrosine-kinase (RTK)EGFR is low in normal prostate, but increases in prostate cancer. This receptor is significantly up-regulated as tumors progress into higher grade, androgen-insensitive and metastatic lesions. The up-regulated receptors could serve as targets for novel selective anti-cancer drugs, e.g. antibodies and tyrosine kinase inhibitors. Radionuclide imaging of RTK can facilitate patient stratification and monitoring of anti-RTK therapy of prostate cancer. The goal of the study was to evaluate binding and cellar processing of radiolabeled EGFRtargeting conjugates by prostate cancer cell lines. Receptor expression of EGFR was studied in three prostate cancer cell lines: DU145 (brain metastasis of PC, hormone insensitive), PC3 (bone metastasis of PC) and LNCaP (lymph node metastasis of PC, androgen and estrogen receptor positive). Uptake and internalization of anti-EGFR mAbs (cetuximab) and affibody molecule (Z 2377 ) labeled with indium-111 was investigated. EGFR expression on prostate cancer cell lines was clearly demonstrated. Both labelled conjugates 111 In-Z 2377 and 111 In-cetuximab bound to prostate cancer cells in the receptor mediated model. Expression levels were modest but correlate with degree of hormone independence. Internalization of Affibody molecules was relatively slow in all cell lines. Internalization of mAbs was more rapid. The level of EGFR expression in these cell lines is sufficient for in vivo molecular imaging. Slow internalization indicates possibility of the use of non-residualizing labels for affibody molecules.
The data on efficiency of the combined application of pulse laser and ultrasonic therapy in the treatment of patients with rosacea are presented. It is shown that this method has high efficiency concerning all clinical symptoms of a disease, especially, positive effect on a vascular component rosacea.
Mongolia is one of the seven countries in Western Pacific regions with high burden of tuberculosis. The earlier research indicates that there is a difference in the distribution of some epidemiologically important subtypes of the Beijing lineage in Mongolia and adjacent Russian regions.Aim of the research: assessment of genotypic structure of M. tuberculosis (MBT) on the border of Russia and central regions of Mongolia.Materials and methods: The DNAs of 143 clinical isolates of MBT from Russian border (46.2%) and central (53.8%) regions of Mongolia have been genotyped by the 24-locus MIRU-VNTR and RD105/RD207. Strains of the Beijing lineage have been analyzed additionally according to the classification by Merker et al. (2015). Results. The study of MBT in Mongolia indicates significant predominance of strains of the Beijing lineage (79.0%) and Beijing MIT 17 subtype (72.6%). However, the strains of the Beijing subtype W148, widespread in Irkutsk Oblast and Buryatia, have not been noted in Mongolia. According to the classification by M.Merker et al., the majority of studied strains of the Beijing lineage (85.8%) relate to the clonal complexe CC4, infrequently detected in Russian border regions. Statistically significant differences between distribution of clonal complexes among border with Russia and central regions of Mongolia have not been detected.Conclusions. Strains of the clonal complex CC4 of Beijing lineage dominate in central and border to Russia regions of Mongolia, this allows assuming that the different geographical regions were sources of MBT strains, prevalent in Mongolia and adjacent Russian regions.
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