Aim:To investigate the level of knowledge among high school and university students about the human papillomavirus (HPV) and its vaccine. Methods: Our study included 3039 Grade 9-12 students from 12 high schools and university students enrolled in medical and non-medical departments. The students completed a 30-item questionnaire, which included three demographic items (sex, age and grade) and measured knowledge regarding HPV, HPV vaccine, smear test and cervical cancer. Students responded by selecting one of the choices "true/false/I don't know." Results: One thousand seven hundred and forty eight high school students (57.5%) and 1291 university students (42.5%) completed the questionnaire. One thousand three hundred and fourty one students were male (44.1%) and 1698 were female (55.9%). Mean age was 18.8 (min: 14 max: 33). The university group had significantly more correct responses (P = 0.001). The high school group had significantly more unknown responses (P = 0.001). In both groups, female and male students had comparable correct responses (High school; female: 25.28%, male: 24.45%, P = 0.42; University; female: 62.46%, male: 63.09%, P = 0.35). While medical students had more correct responses than both nursing students and students from non-medical university departments, nursing students had more correct responses than students from the non-medical university departments (P = 0.001). Conclusion: Knowledge about HPV is limited in adolescence, which marks the beginning of sexual activity. Educational programs targeted at adolescents are needed to negate the increasing rate of HPV-related gynecological cancers in our population. The HPV vaccine should urgently be introduced to the routine immunization schedule.