During plant growth, dividing cells in meristems must coordinate transitions from division to expansion and differentiation, thus generating three distinct developmental zones: the meristem, elongation zone and differentiation zone 1 . Simultaneously, plants display tropisms, rapid adjustments of their direction of growth to adapt to environmental conditions. It is unclear how stable zonation is maintained during transient adjustments in growth direction. In Arabidopsis roots, many aspects of zonation are controlled by the phytohormone auxin and auxin-induced PLETHORA (PLT) transcription factors, both of which display a graded distribution with a maximum near the root tip 2-12 . In addition, auxin is also pivotal for tropic responses 13,14 . Here, using an iterative experimental and computational approach, we show how an interplay between auxin and PLTs controls zonation and gravitropism. We find that the PLT gradient is not a direct, proportionate readout of the auxin gradient. Rather, prolonged high auxin levels generate a narrow PLT transcription domain from which a gradient of PLT protein is subsequently generated through slow growth dilution and cell-to-cell movement. The resulting PLT levels define the location of developmental zones. In addition to slowly promoting PLT transcription, auxin also rapidly influences division, expansion and differentiation rates. We demonstrate how this specific regulatory design in which auxin cooperates with PLTs through different mechanisms and on Reprints and permissions information is available at www.nature.com/reprints.
Wood originates from cell proliferation of the vascular cambium. Xylem (i.e. wood) is produced inside and phloem outside of the cambium 1. Morphogenesis in plants is typically coordinated by organiser cells which direct the adjacent stem cells to undergo programmed cell division and differentiation. It is unknown where the vascular cambium stem cells are located and whether the organiser concept applies to the cambium 2. Here, we combine lineage tracing and molecular genetic studies in Arabidopsis thaliana roots to show that cells with xylem identity direct adjacent vascular cambial cells to divide and function as stem cells. Thus, these xylem identity cells constitute an organiser. A local maximum of the phytohormone auxin and consequent expression of class III homeodomain-leucine zipper (HD-ZIP III) transcription factors promote xylem identity and cellular quiescence of the organiser cells. Additionally, the organiser maintains phloem identity cell nonautonomously. Consistent with this dual function of the organiser cells, xylem and phloem originate from a single, bifacial stem cell in each radial cell file, thus confirming the classical theory of a uniseriate vascular cambium 3. Ectopically activated high auxin signalling clones differentiate as xylem vessels, and induce cell divisions and expression of cambial and phloem markers in the adjacent cells, suggesting that local auxin signalling maximum is sufficient to specify a stem cell organiser. Although vascular cambium has a unique function among plant meristems, its stem cell organiser shares features with the root and shoot meristem organisers.
While apical growth in plants initiates upon seed germination, radial growth is only primed during early ontogenesis in procambium cells and activated later by the vascular cambium 1 . Although it is not known how radial growth is organized and regulated in plants, this system resembles the developmental competence observed in some animal systems, in which pre-existing patterns of developmental potential are established early on 2,3 . Here we show that the initiation of radial growth occurs around early protophloem sieve element (PSE) cell files of the root procambial tissue in Arabidopsis. In this domain cytokinin signalling promotes expression of a pair of novel mobile transcription factors, PHLOEM EARLY DOF (PEAR1, PEAR2) and their four homologs (DOF6, TMO6, OBP2 and HCA2), collectively called PEAR proteins. The PEAR proteins form a short-range concentration gradient peaking at PSE and activating gene expression that promotes radial growth. The expression and function of PEAR proteins are antagonized by well-established polarity transcription factors, HD-ZIP III 4 , whose expression is concentrated in the more internal domain of radially non-dividing procambial cells by the function of auxin and mobile miR165/166. The PEAR proteins locally promote transcription of their inhibitory HD-ZIP III genes, thereby establishing a negative feedback loop that forms a robust boundary demarking the zone of cell divisions. Taken together, we have established a network, in which the PEAR -HD-ZIP III module integrates spatial information of the hormonal domains and miRNA gradients during root procambial development, to provide adjacent zones of dividing and more quiescent cells as a foundation for further radial growth. Cambial growth in plants is initiated within the procambial tissues of the apical meristems through periclinal (i.e. longitudinal) divisions associated with formation of the vascular tissues xylem and phloem 1 (Extended Data Fig. 1a). It has been established that during procambial development in Arabidopsis roots there are distinct domains for high auxin and cytokinin signalling, which mark the regions for further development of xylem and phloem/procambium, respectively 5-8 . To accurately map the spatial distribution of the periclinal divisions, we established a new nomenclature for the root procambial cells, including PSE-lateral neighbours (PSE-LN) as cells directly contacting both PSE and the pericycle, the outer procambial cells (OPC) as procambial cells adjacent to the pericycle but not contacting PSE, and SE-internal neighbours (PSE-IN) as cells located internal to and directly contacting PSE (Fig. 1a). Both the PSE cell and PSE-LN showed higher activity of periclinal cell division than the OPC and PSE-IN (Fig. 1b, Extended Data Fig. 1b-d and Supplementary Information).We observed virtually no periclinal divisions in metaxylem (MX) and internal procambial cells (IPC) (Fig. 1b). Furthermore, blocking symplastic transport genetically 9 between the PSE and the surrounding cells results in a dramatic reduct...
Autophagic transport to the vacuole represents an endomembrane trafficking route, which is widely used in plants, not only during stress situations, but also for vacuole biogenesis and during developmental processes. Here we report a role in autophagic membrane transport for EXO70B1 -one of 23 paralogs of Arabidopsis EXO70 exocyst subunits. EXO70B1 positive compartments are internalized into the central vacuole and co-localize with autophagosomal marker ATG8f. This internalization is boosted by induction of autophagy. Loss of function (LOF) mutations in exo70B1 cause reduction of internalized autopagic bodies in the vacuole. Mutant plants also show ectopic hypersensitive response (HR) mediated by salicylic acid (SA) accumulation, increased nitrogen starvation susceptibility and anthocyanin accumulation defects. Anthocyanin accumulation defect persists in npr1x exo70B1 double mutants with SA signaling compromised, while ectopic HR is suppressed. EXO70B1 interacts with SEC5 and EXO84 and forms an exocyst subcomplex involved in autophagy-related, Golgiindependent membrane traffic to the vacuole. We show that EXO70B1 is functionally completely different from EXO70A1 exocyst subunit and adopted a specific role in autophagic transport.
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