The Rising energy requirements and atmospheric contaminations by combustion of gases and conventional fuel, has opened avenues for new, safe, effective and more accessible energy sources. Corn is one of the richest sources for the production of ethanol. This research looked the effect of concentration of sulphuric acid on the yield of bio-ethanol produced from the lingocellulosic material corncob which is an alternative over food derived ethanol, consumption of crude oil and environmental pollution. The main objective of this study is to know the best acid concentration to that can used during acid hydrolysis for the production of ethanol from the cellulosic content of corncobs. In this study, different condition was examined as to access their effect for optimum ethanol production. The method used was acid hydrolysis of corncobs with varied acid molarities of 0.4M, 0.6M, 0.8M and 1M. The UV/visible spectrophotometer of 1M H 2 SO 4 has the highest absorbance of 0.447, followed by 0.8M (0.368), 0.6M (0.292) and 0.4M (0.253). The result obtained from the physical parameters measured for each different concentration after fermentation processes of the bio ethanol produced, 1M H 2 SO 4 of the corncobs prepared produced the highest percentage yield (55.5%) of the bio ethanol followed by 0.8M (50.5%), 0.6M (47%) and 0.4M (42%) which was the lowest yield. This has shown that acid hydrolysis at 1M H 2 SO 4 with moderate yeast concentration 3g/20cm 3 at room temperature and atmosphere pressure can be used to improve the production of bio ethanol.
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