The effect of sodium chloride on the production of organic acids during ripening of buffalo milk Cheddar cheese at elevated temperature was evaluated. Buffalo milk Cheddar cheese was produced by adding sodium chloride at the rate of 0, 1 and 2% and stored for ripening at 4C and 12C for 120 days. The cheese was evaluated for organic acids i.e., lactic, acetic, citric, pyruvic, formic, butyric and maleic and mineral contents, including sodium, calcium and potassium at 60 and 120 days of ripening. The results showed that elevated ripening temperature accelerated the production of all organic acids and significantly increased their concentrations. However, during ripening, no effect on minerals profile was observed. High level of salt decreased the production of organic acids during ripening irrespective of ripening temperatures. Hence, it was concluded that elevated ripening temperature and enhanced sodium concentrations showed considerable effect on organic acids production. PRACTICAL APPLICATIONS Worldwide, Cheddar cheese is produced from cow's milk, but buffalo milk ranks at the top in Pakistan's milk production and, being nutritionally rich, is more suitable for cheese. Moreover, cheese ripening is a lengthy process and attempts to shorten the ripening time using a range of systems have had varying degrees of success. Organic acids are the major products of glycolysis during ripening and play an integral role in cheese quality. The addition of salt affects the cheese ripening and hence influences the production of organic acids. The project was designed with greater significance to determine the effect of sodium chloride on production of organic acids during accelerated ripening of buffalo milk Cheddar cheese. Practically, the project concluded that the optimal quantity of salt addition should be 1% in cheese manufacturing and recommended the ripening at 12C for 120 days in buffalo milk Cheddar cheese for better compositional profile.

BackgroundThe emergence of serious issues of multidrug resistance in the past few years have enforced the use of bacteriocins for combating infections. Threat posed to public health by various multidrug resistant (MDR) organisms can be resolved by discovering new antimicrobial proteins with broad spectrum of inhibition.ResultsIn the current study, Bacteriocin (BAC-IB17) produced by Bacillus subtilis KIBGE-IB17 is found to be effective against different strains of methicillin resistant Staphylococcus aureus (MRSA). The approximate molecular mass of BAC-IB17 is 10.7 kDa. This unique bacteriocin is found to be highly thermostable and pH stable in nature. It also showed its stability against various heavy metals, organic solvents, surfactants and proteolytic enzymes. Amino acid profile of BAC-IB17 clearly showed that this protein mainly consists of non-polar and basic amino acids whereas; some acidic amino acids were also detected. Sequence of first 15 amino acid residues obtained from N-terminal sequencing of BAC-IB17 were NKPEALVDYTGVXNS.ConclusionsThe anti-MRSA property of purified bacteriocin may be used to prevent the spread of MRSA infections. Remarkable features of BAC-IB17 suggests its applications in various pharmaceutical and food industries as it can function under a variety of harsh environmental conditions.

Pakistan is among the nations with a high intake of trans‐fatty acids (TFAs), a major dietary risk factor of noncommunicable diseases (NCDs). Efforts are underway in the country to eliminate industrially produced TFAs from the food supply in keeping with the priority targets of the World Health Organization (WHO) for 2019‐2023. We reviewed the TFA content reported in industrially produced foods and discuss the regulatory landscape for TFAs, to facilitate the required policy changes in Pakistan and ultimately eliminate the TFA burden from industrial food products. The study components include review of published research and product labels as well as review of prevailing food regulations for TFA‐related information. A set of recommendations was also prepared to eliminate TFAs from Pakistan after national consultation workshops conducted in year 2019. Vanaspati ghee (partially hydrogenated vegetable oil), bakery shortening, hard margarines, and fat spreads are identified as the major sources of TFAs. Federal and provincial food authorities have recently established the limits for TFAs in few products; however, the TFA regulations are insufficient and not in line with global best practices. This study informs a comprehensive national strategy for TFA elimination based on knowledge of TFA prevalence associated regulatory control. We recommend to (1) promote actions toward replacement of traditional vanaspati ghee/bakery fats with healthier alternatives; (2) develop and implement best regulatory practices in line with WHO recommendations; and (3) amend food labeling laws so that clear information will be provided to inform consumers healthy food choices.

The objectives of current study were to evaluate the effect of botanical extracts as antioxidants on amino-and fatty acids profile of broiler meat. In total, 200 one-day-old broiler chicks were divided according to a completely randomized design into eight dietary treatments with five replicates of five chicks each. Dietary treatments were: A-positive control (PC) with antibiotics and synthetic antioxidants, B-negative control (NC), C-NC+Trigonellafoenum-graecum, D-NC+Nigella sativa seed, E-NC+Violaodorata, F-NC+Trachyspermumammi, G-PC+Trachy spermumammi+Trigonellafoenum-graecum+Violaodorata, and H-NC+ Trachyspermumammi, Trigonellafoenum-graecum and Viola odorata. Meat amino-and fatty acid contents were analyzed after slaughter at 35 days of age. Data were submitted to one-way analysis of variance and means were compared by Duncan's Multiple Range test. The levels of histidine, isoleucine, leucine, lysine and threonine were significantly higher (p≤0.05) in the H, F, E, H and F groups respectively. Arginine, tryptophan, valine, methionine, and phenylalanine were significantly higher (p≤0.05) in the C, F, E, G and H groups. Total essential amino acid levels were highest (40.36%) in C group (p≤0.05). The meat of H broilers presented significantly higher (p≤0.05) unsaturated fatty acids (54.8%) and omega-3 contents (2.77%) and the lowest saturated fatty acid content (44.8%). Group F present the highest linoleic acid and omega-6 levels and group H the highest (p≤0.05) arachidonic and eicosapentaenoic acid (EPA) contents. It was concluded that botanical extracts aid health-beneficial meat production and the simultaneous inclusion of Trachyspermumammi, Trigonellafoenum-graecum, and Viola odorata in broiler diets is a more suitable option than the other dietary regimes.

The fruits G. asiatica, E. jambolana and C. carandas were extracted with methanol and the extracts were partitioned into four polyphenolic fractions: Flavanols, flavonols, phenolic acids and anthocyanins using solid phase extraction. Each fraction was then analyzed for total phenolics, flavonoids, antioxidant activity by DPPH, β-carotene-linoleic acid assay and total reducing power. Total phenolics ranged 6. 64-107, 56.20-398, 33.38-315 and 20-201 mg/100 g in phenolic acid, flavanol, flavonol and anthocyanin fractions respectively of these fruits which explained the order of their antioxidant activity and reducing power as flavanols > flavonols > anthocyanins > phenolic acid. Total phenolics were highest in E. jambolana fractions (107-398 mg/100 g) but the maximum antioxidant activity (62-85% in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 58-89% in β-carotene-linoleic acid assay) was shown by the fractions in G.asiatica (total phenolics, 67-151 mg/100 g). At all concentrations, the DPPH scavenging activity of flavanol fraction in G. asiatica (85% at 20 ppm) was comparable to BHA (89%).