Nono is a spontaneously fermented yoghurt-like milk product consumed is a staple food commodity in parts of the Sub-Saharan West Africa. Nono is usually consumed along with 'Fura' as 'Fura da Nono' in Nigeria. Studies on physicochemical and bacteriological qualities were carried out on samples of Nono obtained from 5 different sources in Ado-Ekiti, Nigeria. The Nono samples were found to be nutritious, containing moderate levels of ash, crude fat, crude protein and carbohydrate. The pH of the Nono samples was relatively low (4.04 ±0.04), while the density and specific density were close to that of distilled water at room temperature. Total aerobic plate count of Nono samples was 1.8 ±0.02 × 106 CFU.mL -1 . A total of 15 bacteria species namely Eubacterium nodatum, Bacillus subtilis,
The study focused on the examination of laboratory prepared yoghurts which were fermented with selected starter cultures from commercially sold yoghurt. The starter cultures were molecularly identified (16s rRNA) as Enterococcus lactis, Lactobacillus plantarum, Lactobacillus pentosus, Pediococcuspentosaceus and Enterococcus durans. The isolates were examined for bile tolerance as an indicator of their ability to survive in the gut. The starter cultures were used to produce different yoghurts in the following order: Enterococcus lactis produced yoghurt, L. plantarum and L. pentosus produced yoghurt, Pediococcuspentosaceus produced yoghurt, E. durans produced yoghurt and yoghurt produced with all starter cultures. All yoghurts were examined for nutritional quality (vitamin A, B12 and C content, soluble and casein bound magnesium and calcium and proximate nutrient composition). At p ≤0.05, there was statistical significant difference in the nutritional content with P. pentosaceus contained yoghurt, E. durans contained yoghurt and yoghurt produced with a combination of all isolates recording the highest nutritional values and the lowest was observed with the control. Safety tests such as haematology and histology were carried out on wistar rats. After 7 days of feeding the rats in groups with the different yoghurts and a control without yoghurt, there were marked improvements in the red blood cell counts, white blood cell counts but no significant difference in the differentials at p ≤0.05. The isolates were also observed to have no disruptive effect on the morphology and structure of the small intestine. Overall, the use of these lactic acid bacteria strains showed immense benefits in their use as starter cultures and the study demonstrated safety of the final products for consumption.
Indigenous bacteria and fungi were isolated from soil obtained from a metal scrap dumpsite in Ibadan, Nigeria. Soil sample analyses showed presence of calcium, iron, magnesium, zinc, manganese and copper. A selective isolation of microorganisms was done using heavy metal constituted growth media at concentration of 25 mg/L. Lactobacillus casei was isolated from cadmium and lead composed growth media, Corynebacterium xerosis and Corynebacterium kutsceri from nickel composed growth media. Aspergillus niger and Histoplasma capsulatum were isolated from growth media composed of nickel and lead respectively at a 25 mg/L concentration. Growths were observed for all isolates at 50mg/L, 100mg/L and 400mg/L composed growth media. A 7 day bio-treatment process with isolates for uptake of heavy metals from growth media solution at 50 mg/L concentration of heavy metal was done. Histoplasma capsulatum, Aspergillus fumigatus and Aspergillus niger reduced the heavy metal concentrations in lead, nickel and cadmium to 6, 18.12 and 12.45 mg/L respectively. Lactobacillus casei, and C. xerosis reduced the heavy metal concentration of cadmium, lead and nickel to 13.55, 33, 25, 22.38, 15.45 and 29.11 mg/L respectively. These microorganisms reduced the initial concentration of heavy metals and could thus be used for bioremediation processes.
The aim of the study was to investigate the bioethanol production potentials by indigenous bacterial isolates screened for amylolytic and cellulolytic activities. Cassava peels were obtained from local 'garri' producers, while corn grains and corn cobs were gotten from retailers within the Erinfun farm settlement along Afe Babalola University Road, Ado-Ekiti, Ekiti State, Nigeria on June 4, 2015. The substrates (corn flour, corn cob and cassava peels) were sundried for 5 days and ground into fine powder using a hammer mill and analyzed for proximate nutrient composition and reconstituted to a 5% concentration in water before fermentation. The fermentation was set up for 20 days using single cultures of bacteria and co-cultures of bacteria and yeast selectively isolated and screened for amylolytic and cellulolytic activities. Parameters such as pH, total titratable acidity, reducing sugar and optical density were taken at intervals of 5 days and ethanol production was analyzed at the end of fermentation. Three bacterial isolates (Bacillus macerans, Bacillus subtilis and Micrococcus varians) yielded ethanol in all the feed stocks. The highest percentage ethanol yield was observed in the fermentation of corn flour and corn cobs with B. macerans which were 3.6 ± 0.009 and 3.5 ± 0.009, respectively. There was reduction in the pH and total titratable acidity and increase in the reducing sugar content and optical density. B. macerans exhibited the highest average ethanol production (3.5±0.07%). A proximate analysis of the feed stocks showed presence of nitrogen, protein, fat, carbohydrate and ash. This study investigated the potentials of wastes (cassava peels and corn cob) used as feed stocks for bio-ethanol production, in substantially replacing major food crops (corn) as a case study.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.