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Objectives: The objective of this study was to evaluate the performance of the Boditech i-CHROMA™ point-of-care testing (POCT) method for the quantification of serum CReactive Protein (CRP) and Urine Microalbumin (MAU) compared well with the traditional Abbott Architect Ci8200 laboratory method. Design and Methods:Serum samples of CRP (n=44) and urine samples for MAU estimation (n=25) were analysed at the Homerton University Hospital using the routine laboratory method (Abbott Architect Ci8200) and the Boditech i-CHROMA™ POCT method. Results:The i-CHROMA™ CRP and MAU method showed good correlation with that of the Abbott Architect Ci8200: r 2 =0.905 and r 2 =0.987 respectively. Overall, there was a slight negative bias seen with the i-CHROMA™ CRP method (bias=-8.15, bias difference=-9.05%) and a negative bias seen with the i-CHROMA™ MAU method (bias=35.84, bias difference=-27.88%). Conclusion:The Boditech i-CHROMA™ POCT method provide a reliable measurement of CRP and MAU and compared well with the traditional Abbott Architect Ci8200 laboratory method.

This work is licensed under Creative Commons Attribution 4.0 License AJBSR.MS.ID.000859.

The estimation of serum alpha-fetoprotein (AFP) is useful in the diagnosis and monitoring of primary hepatocellular carcinoma, hepatoblastoma, non-seminomatous testicular germ cell tumours and other germ cell tumours. The iCHROMA™ AFP is a lateral flow chromatography, fluorescence immunoassay (FIA) for the quantitative determination of AFP in serum or plasma. In this study, the Boditech iCHROMA™ AFP assay had a very good precision of 9.8%. It showed a very good correlation with the following 12 methods: Abbott Architect (r2 = 0.9705), BioMerieux VIDAS (r2 = 0.9717), Roche Cobas 6000/8000 (r2 = 0.9738), Siemens Centaur XP/XPT/Classic (r2 = 0.9654), Siemens/DPC/Immulite 2000/2500 (r2 = 0.9673), Siemens/DPC/Immulite 1000 (r2 = 0.9670), Beckman Dxl 600/800 (r2 = 0.9676), Roche Elecsys (r2 = 0.9683), Roche Cobas 4000/e411 (r2 = 0.9688), Roche Modular E170 (r2 = 0.9692), SNIBE Maglumi (r2 = 0.9457) and Ortho Vitros 3600/5600/ECi (r2 = 0.9714). In summary, the iCHROMA™ AFP, a rapid point of care test method, has a within-run precision value of less than 10% and excellent correlations with traditional laboratory methods. There is a consistent overestimation with the iCHROMA™ method, which must be taken into consideration when setting a reference range.

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