Taking soy-based food supplements for menopausal symptoms by women may reduce the risk of cancer. Therefore, the interaction between nucleic acids (or their constituents) and ingredients of the supplements, e. g., isoflavone glucosides, on the molecular level, has been of interest with respect to cancer therapy. In this work, the interaction between isoflavone glucosides and G-tetrads, namely [4G + Na] + ions (G stands for guanosine or deoxyguanosine), were analyzed by using electrospray ionization-collision induced dissociation-mass spectrome-try (ESI-CID-MS) and survival yields method. The strength of isoflavone glucosides-[4G + Na] + interaction in the gas phase was determined from E com50 -the energy required to fragment 50 % of selected precursor ions. Glycitin-[4G + Na] + interaction was found to be the strongest, and the interaction between isoflavone glucosides and guanosine tetrad was established to be stronger than that between isoflavone glucosides and deoxyguanosine tetrad.
Gas-phase
decompositions of magnesium complexes with adenosine-5′-triphosphate
(ATP) and adenosine-5′-diphosphate (ADP) were studied by using
electrospray ionization-collision-induced dissociation-tandem mass
spectrometry, in the negative ion mode. The loss of internal ribose
residue was observed and was found to occur directly from the [ADP-3H+Mg]− ion. The occurrence of this process indicates the
presence of a strong phosphate-Mg-adenine interaction. The performed
quantum mechanics calculations confirmed the occurrence of this interaction
in the [ADP-3H+Mg]− ion, namely the presence of
Mg–N7 bond and hydrogen bond between the phosphate oxygen atom
and amino group. Although the finding concerns the gas phase, it indicates
that phosphate-Mg-adenine interaction may be also of importance for
biological processes. The loss of an internal ribose residue was also
observed for calcium and zinc complexes with ATP/ADP as well as for
magnesium complexes with guanosine-5′-triphosphate (GTP) or
guanosine-5′-diphosphate (GDP). Therefore, it is reasonable
to conclude that the presence of the phosphate-metal-nucleobase interaction
is a feature of gas phase [NDP-3H+metal]− ion (NDP,
nucleoside-5′-diphosphate) and may also be important for biological
processes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.