Summary.Major leucocyte subpopulations were isolated from peripheral blood of healthy donors, and patients with chronic myeloid leukaemia (CML) and chronic lymphoid leukaemia (CLL). In vitro UV irradiation was performed at the wavelength of 257 nm (UVC band). DNA double-stranded breaks (DNAdsbs) were detected immediately after UVirradiation, by means of agarose gel electrophoresis. Cell viability was estimated after 18 h in culture, as relative numbers of residual non-apoptotic cells. Evaluation of the dose-response curves revealed that normal CLL lymphoid cells showed only moderate damage after UV-irradiation, as assessed by DNAdsbs and cell viability criteria. However, normal granulocytes and myeloid blasts from CML patients expressed a sharp increase in DNAdsbs, even at lower doses of UV-radiation. UV-induced amplification of endogenous oxidative systems (e.g. NADPH-dependent oxidase) is suggested as a probable reason for enhanced DNA breakage and apoptosis in cells of the granulocytic lineage.
Aim. In this study, we carry out a problem and critical analysis of the process of implementing the 5S lean manufacturing methodology in the Healthcare System of the Russian Federation.Materials. 33 Russian and foreign literature sources indexed in the RSCI, Scopus, Web of Science and PubMed databases were reviewed.Results. This article scrutinizes 5 consecutive steps of the 5S lean manufacturing method for organizing a workplace. This method, which is based on interrelated principles, is being actively introduced into the Russian Healthcare System with the purpose of identifying, reducing and preventing various types of losses.Conclusion. It is shown that the practical realization of the 5S method is hampered by the specifi cs of medical personnel work and the peculiarities of legislative regulation in RF medical organizations. The application of the 5S methodology, taking into account the existing regulatory framework in the fi eld of healthcare, can increase the manageability of workplaces and create conditions for the effective work of medical personnel.
The article presents the results of a review of literature on the possibility of visualization in the health care system of the Russian Federation as one of the main methods of implementation of lean production. The historical aspect of the use of various imaging techniques in medicine is reflected in detail, as well as the advantages of some visualization tools in the organization of medical care. The existing legal and methodological framework for the use of visualization in the activities of medical organizations of various profiles is analyzed. Search publications carried out on the bases e-library, Medline, Scopus, Pub Med, The Cochrane Library and RINC.
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