The intrinsic slow growth of nitrifying bacteria and their high sensitivity to environmental perturbations often result in cell growth inhibition by toxicants. Nanoparticles are of great concern to the environment because of their small size and high catalytic properties. This work sought to determine size-dependent inhibition by Ag nanoparticles and evaluate the relationship between the inhibition and reactive oxygen species (ROS). Nanoparticles with an average size range of 9-21 nm were synthesized by varying the molar ratios of BH4-/Ag+ in the solution. The resulting ROS generation was quantified in the presence and absence of the bacteria while the degree of inhibition was inferred from specific oxygen uptake rate measurements, determined by extant respirometry. By examining the correlation between nanoparticle size distribution, photocatalytic ROS generation, intracellular ROS accumulation, and nitrification inhibition, we observed that inhibition to nitrifying organisms correlated with the fraction of Ag nanoparticles less than 5 nm in the suspension. It appeared that these size nanoparticles could be more toxic to bacteria than any other fractions of nanoparticles or their counterpart bulk species. Furthermore, inhibition by Ag nanoparticles as well as other forms of silver (AgCl colloid and Ag+ ion) correlated well with the intracellular ROS concentrations, but not with the photocatalytic ROS fractions. The ROS correlations were different for the different forms of silver, indicating that factors other than ROS are also important in determining nanosilver toxicity.
Although microbes directly accepting electrons from a cathode have been applied for CO2 reduction to produce multicarbon-compounds, a high electron demand and low product concentration are critical limitations. Alternatively, the utilization of electrons as a co-reducing power during fermentation has been attempted, but there must be exogenous mediators due to the lack of an electroactive heterotroph. Here, we show that Clostridium pasteurianum DSM 525 simultaneously utilizes both cathode and substrate as electron donors through direct electron transfer. In a cathode compartment poised at +0.045 V vs. SHE, a metabolic shift in C. pasteurianum occurs toward NADH-consuming metabolite production such as butanol from glucose (20% shift in terms of NADH consumption) and 1,3-propandiol from glycerol (21% shift in terms of NADH consumption). Notably, a small amount of electron uptake significantly induces NADH-consuming pathways over the stoichiometric contribution of the electrons as reducing equivalents. Our results demonstrate a previously unknown electroactivity and metabolic shift in the biochemical-producing heterotroph, opening up the possibility of efficient and enhanced production of electron-dense metabolites using electricity.
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