No abstract

Piperonyl butoxide (PBO), a methylenedioxyphenyl compound, is primarily used as a synergist in combination with space spray, residual and admixture products for the control of insect pests in or around domestic and commercial premises, especially food preparation areas. Also, tetramethrin is known as a knockdown agent on target organism and it is generally used with piperonyl butoxide. In this study, effects of piperonyl butoxide and tetramethrin combinations on biological activities of synthetic pyrethroids, cypermethrin, deltamethrin, and permethrin against different housefly (Musca domestica Linnaeus, 1758) populations were evaluated. In addition, the biological efficiency of the insecticides used in the study, insecticide + PBO and insecticide + PBO + tetramethrin combinations, against the WHO standard sensitive housefly population and housefly populations collected from different parts of Turkey were compared.Results showed that PBO extensively promoted the ratio of knockdown and killing effect values of the insecticides. The results also indicated that PBO and PBO + tetramethrin combinations moderately reduced the knockdown effect times of all formulation in all housefly populations. The knockdown effect times were more decreased by insecticide + PBO + tetramethrin combinations than insecticides that are used alone and insecticide + PBO combinations. Pest control, insect control, synergism, Turkey insects, fight against insect vectors of disease

The aim of this study was the optimisation of a multi-analyte method for the analysis of primary aromatic amines (PAAs) from napkins in order to support official controls and food safety. We developed a UHPLC-MS/MS method for the simultaneous determination of 36 toxicologically relevant PAAs for paper and board. Good regression coefficients of the calibration curves in a range of 0.992–0.999 and reproducibilities in a range of 2.3–15% were obtained. Limits of detections (LODs) were in the range of 0.03–1.4 µg l–1 and recoveries were in a range of 21–110% for all the amines. A total of 93 coloured paper napkin samples from different European countries were bought and extracted with water to determine the PAAs. The results showed that 42 of 93 samples contained at least one PAA. More than half of the detected PAAs are considered as toxic, carcinogenic or probably carcinogenic to humans by the International Agency for Research on Cancer (IARC), or are classified as such in the European Union legislation on chemicals. Summed concentrations of PAAs in seven samples were higher than 10 µg l–1, the limit of summed PAA in the European Union plastic food contact material regulation. Also, eight PAAs, classified as Category 1A and 1B carcinogen in the European Union legislation of chemicals, were detected at concentrations higher than 2 µg l–1, exceeding the limit proposed by the Federal Institute for Risk Assessment in Germany. Aniline (n = 14) was most frequently present in higher concentrations followed by o-toluidine, o-anisidine, 2,4-dimethylaniline and 4-aminoazobenzene. Red, orange, yellow and multicoloured paper napkins contained the highest concentrations of total PAAs (> 10 µg l–1). Although the European Union has not harmonised the legislation of paper and board materials and, thus, there is no specific migration limit for PAAs from paper napkins, the present study showed that coloured paper napkins can contain toxic and carcinogenic PAAs at concentrations that are relevant for monitoring.

Forty-seven fish (24 endemic Alburnus tarichi, Güldenstädt, 1814; 8 Capoeta capoeta, Guldenstaedt, 1772; 15 mirror carp Cyprinus carpio, L., 1758) and 13 mussel (Unio stevenianus, Krynicki, 1837) samples, with 10 specimens per sample, were collected from Van Lake, Turkey, and rivers flowing into it. Gamma-HCH was detected in 21 Alburnus tarichi samples (56.57 ng/g ± 22.18 ng/g) and in two Capoeta capoeta samples (27.6 ng/g and 36.45 ng/g). Beta-HCH was detected in 8 Alburnus tarichi samples (24.95 ng/g ± 4.42 ng/g) and in two mussel samples (101.25 ng/g and 129.44 ng/g). HCB was found in one Alburnus tarichi sample (14.4 ng/g) and one mussel sample (181.25 ng/g). The compound 4,4'-DDE was detected in 21 Alburnus tarichi samples (87.13 ng/g ± 32.23 ng/g), in 9 mirror carp samples (304.82 ng/g ± 100.76 ng/g) and one mussel sample (149.31 ng/g). PCB 28 was detected in one Alburnus tarichi (19.46 ng/g) sample and PCB 101 was found in one Capoeta capoeta (60.16 ng/g) sample. PCB 118 was detected in one mirror carp sample (277.5 ng/g) and in two Capoeta capoeta samples (43.77 and 54.38 ng/g). PCB 128 was detected in only one Capoeta capoeta sample (141.48 ng/g). It is concluded that (i) efforts should be made to reduce contamination of aquatic environments by these compounds and that (ii) their levels in fishery products from Van Lake and connected streams should be monitored and publicly reported on a regular basis.

Cisplatin is one of the commonly used anticancer drugs and nephrotoxicity limits its use. The aim of this study is to investigate the possible protective effect of creatine supplementation on cisplatin-induced nephrotoxicity. Sixty male Sprague-Dawley rats were divided into three groups: Group I: Cisplatin (n ¼ 20) (7 mg/kg cisplatin intraperitoneal (i.p.) single dose), group II: Cisplatin + creatine monohydrate (n ¼ 20) (7 mg/kg cisplatin i.p. single dose and 300 mg/kg creatine p.o. daily for 30 days starting on first day of cisplatin injection), group III: Control group (n ¼ 20) (Serum physiologic, 2.5 mL/kg i.p.). Sacrifications were performed at first week and 30th day. Blood urea nitrogen (BUN) and serum creatinine levels, histopathological evaluation, mitochondrial deoxyribonucleic acid (mtDNA) common deletion rates, and body weights of rats were evaluated. A significant decrease in body weight, higher values of kidney function tests, histopathological scores, and mtDNA deletion ratios were observed in group I compared to control group at days 7 and 30 (p50.05). In group II, there was a slight decrease in body weight at same days (p ¼ 0.931 and 0.084, respectively). Kidney function tests, histopathological scores, and mtDNA common deletion ratios were statistically better in group II than group I at 7th and 30th day (p50.05). Although creatine significantly reversed kidney functions and pathological findings, this improvement was not sufficient to reach normal control group's results at days 7 and 30. In conclusion, the present study demonstrates that creatine administration is a promising adjuvant protective drug for reducing nephrotoxic effect of cisplatin.

No abstract

The aim of this study was to evaluate dichlorvos toxicity in terms of nitro-oxidative stress by determining 3-nitrotyrosine (3-NT) levels in the fore, mid, and hindbrain regions in acutely exposed rats. Male SpragueDawley rats were randomly allocated to three groups of eight. Group 1 was administered a single intraperitoneal dichlorvos dose of 1.8 mg kg -1 (0.1xLD 50 ) and group 2 a dose of 9 mg kg -1 (0.5xLD 50 ). The control group received 0.5 mL saline solution via the same route. 3-NT and tyrosine (TYR) levels were measured using high performance liquid chromatography with a photodiode array detector (HPLC-PDA) and expressed as a ratio of 3-NT to TYR. The 3-NT/1000 TYR ratios increased significantly in the fore-, mid-and hindbrains of the exposed groups compared to control (p<0.01). In the forebrain, the increase was also significant between the treated groups. Our study has confirmed that acute exposure to dichlorvos leads to nitro-oxidative stress in the brain and that 3-NT may play a role in the mechanism of dichlorvos neurotoxicity.

The aim of this study was the determination of plasma concentrations of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) in cats and dogs and evaluation of their prevalence and possible effects. The concentrations of nine OCPs, such as α-hexachlorocyclohexane (HCH), β-HCH, γ-HCH, hexachlorobenzene (HCB), aldrin, 2,4'-dichlorodiphenyltrichloroethane (2,4'-DDT), 4,4'-DDT, 2,4'-dichlorodiphenyldichloroethylene (2,4'-DDE) and 4,4'-DDE and 16 PCBs (PCB-28, -52, -70, -74, -81, -99, -101, -118, -138, -153, -156, -170, -180, -183, -187 and -208) were evaluated in the plasma samples of pet cats ( n = 15) and dogs ( n = 21). The concentrations of OCPs ranged from 1.12 ng g lipid weight (lw) to 7.65 ng g lw in cats and from 1.25 ng g lw to 6.79 ng g lw in dogs. In addition, mean PCB levels were 0.58-5.66 and 0.52-6.62 ng g lw in cats and dogs, respectively. β-HCH, γ-HCH and PCB-138 levels were significantly higher in dogs ( p < 0.05). As far as could be determined, OCPs and PCBs were detected in the plasma samples of domestic cats and dogs in Turkey for the first time. Their concentrations were similar to those reported in earlier studies abroad. However, in contrast to other research, the levels of some OCPs were higher in dogs than in cats. It is concluded that, because of their high prevalence and potential health effects in animals and humans, OCP and PCB levels should be monitored systematically in domestic cats and dogs.