Strains were grown using standard growth conditions on NGM agar at 20 0 C on Escherichia coli OP50, unless otherwise stated (Brenner, 1974).Neuroanatomical reporter strains used -mgIs70 Is ;LGIV zdIs13:Is . Detailed strain information is detailed in Table S2. Molecular cloning Ppdi-1::pdi-1 cDNA rescue constructThe Ppdi-1::pdi-1 cDNA rescue construct was generated by cloning the 841 bp pdi-1 promoter with SphI-SmaI into the pPD49.26 vector and then the pdi-1 cDNA using NheI-SacI. Pdpy-7::pdi-1 cDNA rescue constructThe Pdpy-7::pdi-1 cDNA rescue construct was generated by cloning pdi-1 cDNA with NheISacI into a dpy-7 expression vector. Pdpy-7::pdi-1 cDNA -HEEL rescue constructThe Pdpy-7::pdi-1 cDNA -HEEL rescue construct was generated using site-directed mutagenesis to remove the 12 nucleotides that encode HEEL at the C-terminus of the PDI-1 protein. Pegl-20::pdi-1 cDNA rescue constructThe Pegl-20::pdi-1 cDNA rescue construct was generated by cloning the 1910 bp egl-20 promoter with SphI-SmaI into the pPD49.26 vector containing the pdi-1 cDNA. Plin-44::pdi-1 cDNA rescue constructThe Plin-44::pdi-1 cDNA rescue construct was generated by cloning the 1150 bp lin-44 promoter with SphI-SmaI into the pPD49.26 vector containing the pdi-1 cDNA.
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