Legionella is a ubiquitous water environmental organism. Isolation/detection of Legionellae has been reported worldwide. However, there is no reported isolation of Legionellae in countries in Africa with the exception of South Africa. The aim of this study was to survey the surface, ground waters and air conditioner water systems (ACWs) in Osogbo, Nigeria for the presence of Legionella pneumophila by cultural isolation method. A total of 313 water samples from the streams (surface water), wells (groundwater) and ACWS were cultured on buffered charcoal yeast extract (BCYE) medium. The isolates were identified by Gram staining techniques, biochemical tests and demonstration of pore forming activity. Polymerase chain reaction for amplification of 0.52 kb fragment of the part of dot/icm region of L. pneumophila was used for the final identification. The results obtained showed that the prevalence of L. pneumophila from streams (8%) was statistically significant when compared to wells (1%) (Chi square, P < 0.05). L. pneumophila was not isolated from any of the water samples taken from air-conditioner water systems. The study concludes that L. pneumophila is present in natural aquatic environment in Nigeria.
The relationship between prostate cancer and syphilis and the relevance of the known risk factors such as age, occupation and physical/social activities of these patients on this relationship was determined. Blood samples were collected by convenience sampling method from 132 men (45 -89 yrs) attending the Cancer
This work was carried out in collaboration between all authors. Author ODO designed the study, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Author AOAT was also involved in the design of the study and statistical analysis. Authors YRA and OAS managed the analyses of the study. Author YRA managed the literature searches. Author AWM provided the protocol for the study and was involved in the design and proof read the final draft. All authors read and approved the final manuscript.
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