Mesoporous hydroxyapatites were synthesized at room temperature using casein as a template, and key experimental factors, such as casein concentration, pH and extraction of casein in the final solids by washing and thermal treatment, were systematically investigated. The X-Ray Diffraction (XRD) patterns confirmed the synthesis of well-crystallized hydroxyapatite. The N adsorption/desorption isotherms were in agreement with the formation of mesoporous hydroxyapatite with a maximum surface area of 106mg. Infrared spectroscopy and thermogravimetry analysis were performed to investigate the extraction of casein in water in the post-synthesis stage. Pure mesoporous hydroxyapatite exhibited good BSA adsorption capacity higher than the one obtained for conventional hydroxyapatite.
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